Effects of aging on activities of mitochondrial electron transport chain complexes and oxidative damage in rat heart
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21114360
DOI
10.33549/physiolres.932019
PII: 932019
Knihovny.cz E-resources
- MeSH
- Aldehydes metabolism MeSH
- Electron Transport Chain Complex Proteins metabolism MeSH
- Rats MeSH
- Malondialdehyde metabolism MeSH
- Myocardium metabolism MeSH
- Oxidative Stress * MeSH
- Lipid Peroxidation MeSH
- Rats, Wistar MeSH
- Mitochondria, Heart chemistry metabolism MeSH
- Aging metabolism MeSH
- Sulfhydryl Compounds metabolism MeSH
- Electron Transport MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 4-hydroxy-2-nonenal MeSH Browser
- Aldehydes MeSH
- Electron Transport Chain Complex Proteins MeSH
- Malondialdehyde MeSH
- Sulfhydryl Compounds MeSH
Mitochondrial dysfunction and accumulation of oxidative damage have been implicated to be the major factors of aging. However, data on age-related changes in activities of mitochondrial electron transport chain (ETC) complexes remain controversial and molecular mechanisms responsible for ETC dysfunction are still largely unknown. In this study, we examined the effect of aging on activities of ETC complexes and oxidative damage to proteins and lipids in cardiac mitochondria from adult (6-month-old), old (15-month-old) and senescent (26-month-old) rats. ETC complexes I-IV displayed different extent of inhibition with age. The most significant decline occurred in complex IV activity, whereas complex II activity was unchanged in old rats and was only slightly reduced in senescent rats. Compared to adult, old and senescent rat hearts had significantly higher levels of malondialdehyde, 4-hydroxynonenal (HNE) and dityrosine, while thiol group content was reduced. Despite marked increase in HNE content with age (25 and 76 % for 15- and 26-month-old rats, respectively) Western blot analysis revealed only few HNE-protein adducts. The present study suggests that non-uniform decline in activities of ETC complexes is due, at least in part, to mitochondrial oxidative damage; however, lipid peroxidation products appear to have a limited impact on enzyme functions.
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