Characterization of P19 cells during retinoic acid induced differentiation
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21189168
PII: PMR2010A0033
Knihovny.cz E-resources
- MeSH
- Antigens, Surface genetics metabolism MeSH
- Cell Differentiation drug effects genetics MeSH
- Gene Expression MeSH
- Homeodomain Proteins genetics metabolism MeSH
- Immunohistochemistry MeSH
- Embryonal Carcinoma Stem Cells physiology MeSH
- Mice MeSH
- Cell Line, Tumor MeSH
- Tumor Cells, Cultured MeSH
- Eye Proteins genetics metabolism MeSH
- Octamer Transcription Factor-3 genetics metabolism MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Microtubule-Associated Proteins genetics metabolism MeSH
- Repressor Proteins genetics metabolism MeSH
- PAX6 Transcription Factor MeSH
- Basic Helix-Loop-Helix Transcription Factors genetics metabolism MeSH
- Paired Box Transcription Factors genetics metabolism MeSH
- Tretinoin pharmacology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- antigen FORSE-1 MeSH Browser
- Antigens, Surface MeSH
- ASCL1 protein, human MeSH Browser
- Homeodomain Proteins MeSH
- Eye Proteins MeSH
- Octamer Transcription Factor-3 MeSH
- PAX6 protein, human MeSH Browser
- Pax6 protein, mouse MeSH Browser
- Pou5f1 protein, mouse MeSH Browser
- Microtubule-Associated Proteins MeSH
- Repressor Proteins MeSH
- PAX6 Transcription Factor MeSH
- Basic Helix-Loop-Helix Transcription Factors MeSH
- Paired Box Transcription Factors MeSH
- Tretinoin MeSH
The aim of our study was to characterize mouse embryonal carcinoma (EC) cells P19 in different stages of retinoic acid induced neurodifferentiation by two methods, immunocytochemistry and RT qPCR. The characterization of the cells is crucial before any transplantation into any model, e.g. in our case into the mouse brain with the aim to treat a neurodegenerative disease. Specific protein markers (MAP-2, OCT-4, FORSE-1) were detected by immunocytochemistry in the cell cultures. The mRNA expression levels of PAX-6, MASH-1, Brachyury, GATA-4 and AFP were determined by RT qPCR method. HPRT was used as a housekeeping gene. The degree of differentiation can be characterized by expression of analyzed genes. The presence of OCT-4 and FORSE-1 proteins in undifferentiated pluripotent cells and the presence of dendrite specific MAP-2 in neuroprogenitors was detected. The expression levels of PAX-6 and MASH-1 increased and expression of Brachyury decreased during the neurodifferentiation process. The expression levels of GATA-4 and AFP were the highest after induction of differentiation with retinoic acid. Detailed characterization of cells before transplantation experiments can contribute to better understanding of their effect.
