Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21195113
DOI
10.1016/j.molbiopara.2010.12.011
PII: S0166-6851(10)00327-0
Knihovny.cz E-resources
- MeSH
- Antigens, Protozoan genetics MeSH
- Chromosomes chemistry genetics ultrastructure MeSH
- DNA Fingerprinting * methods MeSH
- Fluorescence MeSH
- Genetic Markers MeSH
- Genome MeSH
- Genotype MeSH
- Gene Dosage MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Humans MeSH
- Chromosome Mapping methods MeSH
- Sensitivity and Specificity MeSH
- Serine C-Palmitoyltransferase genetics MeSH
- Trichomonas vaginalis chemistry cytology genetics MeSH
- Trichomonas Infections genetics parasitology MeSH
- Tryptophanase genetics MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Protozoan MeSH
- Genetic Markers MeSH
- Serine C-Palmitoyltransferase MeSH
- Tryptophanase MeSH
The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T. vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome I and II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mapping of the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping.
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