The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T. vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome I and II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mapping of the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping.

Charles University Prague Faculty of Science Department of Parasitology Vinicna 7 12844 Prague Czech Republic

Citace poskytuje Crossref.org

Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system

The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion

Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis

Histone H3 Variants in Trichomonas vaginalis