Genetic mapping of DArT markers in the Festuca-Lolium complex and their use in freezing tolerance association analysis
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Brachypodium genetics MeSH
- Chromosomes, Plant MeSH
- Festuca genetics MeSH
- Adaptation, Physiological genetics MeSH
- Genetic Linkage MeSH
- Genetic Markers * MeSH
- Lolium genetics MeSH
- Humans MeSH
- Chromosome Mapping methods MeSH
- Molecular Sequence Data MeSH
- Polymorphism, Genetic MeSH
- Oryza genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Synteny MeSH
- Freezing * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Genetic Markers * MeSH
Species belonging to the Festuca-Lolium complex are important forage and turf species and as such, have been studied intensively. However, their out-crossing nature and limited availability of molecular markers make genetic studies difficult. Here, we report on saturation of F. pratensis and L. multiflorum genetic maps using Diversity Array Technology (DArT) markers and the DArTFest array.The 530 and 149 DArT markers were placed on genetic maps of L. multiflorum and F. pratensis, respectively, with overlap of 20 markers, which mapped in both species. The markers were sequenced and comparative sequence analysis was performed between L. multiflorum, rice and Brachypodium. The utility of the DArTFest array was then tested on a Festulolium population FuRs0357 in an integrated analysis using the DArT marker map positions to study associations between markers and freezing tolerance. Ninety six markers were significantly associated with freezing tolerance and five of these markers were genetically mapped to chromosomes 2, 4 and 7. Three genomic loci associated with freezing tolerance in the FuRs0357 population co-localized with chromosome segments and QTLs previously identified to be associated with freezing tolerance. The present work clearly confirms the potential of the DArTFest array in genetic studies of the Festuca-Lolium complex. The annotated DArTFest array resources could accelerate further studies and improvement of desired traits in Festuca-Lolium species.
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