Complementary attributes of Festuca and Lolium grasses can be combined in hybrid cultivars called Festuloliums, which are becoming increasingly popular fodder crops and amenity plants. Genomic constitution of commercially available Festuloliums was reported to vary from almost equal representation of parental genomes to apparent lack of one of them based on molecular cytogenetic analyses and screening with a small set of DNA markers, both approaches with limited resolution. Here, we describe the use of the DArTFest array comprising 3,884 polymorphic DArT markers for characterization of genomes in five Festulolium cultivars. In any of the cultivars, the minimum number of informative markers, which discriminated the parental Lolium and Festuca genomes was 361 and 171, respectively. Using the DArTFest array, it was possible to determine hybrid genome constitution at resolution which has never been achieved before and the analysis of a set of randomly selected plants from each cultivar provided information on genetic structure of outcrossing Festulolium cultivars. In addition to a core set of markers typical for each hybrid cultivar, markers occurring at low frequency among the plants within each cultivar were identified. Biological significance of genomic loci associated with the rare markers is yet to be determined. Finally, with the aim to simplify the use of DArTFest arrays to characterize Festuca × Lolium hybrids, various bulking strategies were compared. While all bulks were suitable for identification of hybrids, only bulks of few plants have been found to reveal the rare markers.

Laboratory of Molecular Cytogenetics and Cytometry Institute of Experimental Botany Sokolovská 6 77200 Olomouc Czech Republic

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Theor Appl Genet. 2003 Dec;108(1):25-40 PubMed

Nucleic Acids Res. 2004 Jan 1;32(Database issue):D360-3 PubMed

Ann Bot. 2009 May;103(7):1039-48 PubMed

Genome. 2004 Feb;47(1):57-65 PubMed

Theor Appl Genet. 2006 Aug;113(4):731-42 PubMed

Theor Appl Genet. 2002 Sep;105(4):577-584 PubMed

Proc Natl Acad Sci U S A. 2004 Jun 29;101(26):9915-20 PubMed

Theor Appl Genet. 2009 May;118(8):1549-60 PubMed

Theor Appl Genet. 2005 Jan;110(2):323-36 PubMed

Nucleic Acids Res. 2001 Feb 15;29(4):E25 PubMed

Curr Opin Plant Biol. 2005 Apr;8(2):135-41 PubMed

Theor Appl Genet. 2004 Oct;109(6):1139-46 PubMed

Theor Appl Genet. 1994 Sep;88(8):909-13 PubMed

BMC Genomics. 2009 Oct 15;10:473 PubMed

Genome. 2007 Apr;50(4):373-84 PubMed

Cytogenet Genome Res. 2005;109(1-3):373-7 PubMed

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