Metallochelating liposomes with associated lipophilised norAbuMDP as biocompatible platform for construction of vaccines with recombinant His-tagged antigens: preparation, structural study and immune response towards rHsp90
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21256901
DOI
10.1016/j.jconrel.2011.01.016
PII: S0168-3659(11)00022-8
Knihovny.cz E-resources
- MeSH
- Antigens, Fungal immunology metabolism MeSH
- Coated Materials, Biocompatible metabolism MeSH
- Immunity, Cellular * MeSH
- Candida immunology MeSH
- Chelating Agents chemistry metabolism MeSH
- Dendritic Cells immunology metabolism MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Liposomes MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Nickel immunology metabolism MeSH
- HSP90 Heat-Shock Proteins immunology metabolism MeSH
- Vaccines, Synthetic immunology metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Antigens, Fungal MeSH
- Coated Materials, Biocompatible MeSH
- Chelating Agents MeSH
- Liposomes MeSH
- Nickel MeSH
- HSP90 Heat-Shock Proteins MeSH
- Vaccines, Synthetic MeSH
Hsp90-CA is present in cell wall of Candida pseudohyphae or hyphae-typical pathogenic morphotype for both systemic and mucosal Candida infections. Heat shock protein from Candida albicans (hsp90-CA) is an important target for protective antibodies during disseminated candidiasis of experimental mice and human. His-tagged protein rHsp90 was prepared and used as the antigen for preparation of experimental recombinant liposomal vaccine. Nickel-chelating liposomes (the size around 100nm, PDI≤0.1) were prepared from the mixture of egg phosphatidyl choline and nickel-chelating lipid DOGS-NTA-Ni (molar ratio 95:5%) by hydration of lipid film and extrusion methods. New non-pyrogenic hydrophobised derivative of MDP (C18-O-6-norAbuMDP) was incorporated into liposomes as adjuvans. rHsp90 was attached onto the surface of metallochelating liposomes by metallochelating bond and the structure of these proteoliposomes was studied by dynamic light scattering, AF microscopy, TEM and GPC. The liposomes with surface-exposed C18-O-6-norAbuMDP were well recognised and phagocyted by human dendritic cells in vitro. In vivo the immune response towards this experimental vaccine applied in mice (i.d.) demonstrated both TH1 and TH2 response comparable to FCA, but without any side effects. Metallochelating liposomes with lipophilic derivatives of muramyl dipeptide represent a new biocompatible platform for construction of experimental recombinant vaccines and drug-targeting systems.
References provided by Crossref.org
Targeting Human Thrombus by Liposomes Modified with Anti-Fibrin Protein Binders
