Activation of cumulus cell SMAD2/3 and epidermal growth factor receptor pathways are involved in porcine oocyte-cumulus cell expansion and steroidogenesis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
21520325
DOI
10.1002/mrd.21312
Knihovny.cz E-zdroje
- MeSH
- benzamidy farmakologie MeSH
- C-reaktivní protein metabolismus MeSH
- chinazoliny farmakologie MeSH
- dioxoly farmakologie MeSH
- epidermální růstový faktor metabolismus MeSH
- erbB receptory antagonisté a inhibitory metabolismus MeSH
- folikuly stimulující hormon metabolismus MeSH
- glukuronosyltransferasa antagonisté a inhibitory metabolismus MeSH
- isochinoliny farmakologie MeSH
- kumulární buňky metabolismus MeSH
- kyselina hyaluronová biosyntéza MeSH
- meióza účinky léků MeSH
- molekuly buněčné adheze antagonisté a inhibitory metabolismus MeSH
- myši MeSH
- oocyty enzymologie fyziologie MeSH
- prasata MeSH
- progesteron biosyntéza MeSH
- protein Smad2 antagonisté a inhibitory metabolismus MeSH
- protein Smad3 antagonisté a inhibitory metabolismus MeSH
- pyridiny farmakologie MeSH
- pyrroly farmakologie MeSH
- sérový amyloidový protein metabolismus MeSH
- signální transdukce účinky léků MeSH
- tyrphostiny farmakologie MeSH
- vývojová regulace genové exprese účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide MeSH Prohlížeč
- 6,7-dimethyl-2-(2E)-3-(1-methyl-2-phenyl-1H-pyrrolo(2,3-b)pyridin-3-yl-prop-2-enoyl)-1,2,3,4-tetrahydroisoquinoline hydrochloride MeSH Prohlížeč
- benzamidy MeSH
- C-reaktivní protein MeSH
- chinazoliny MeSH
- dioxoly MeSH
- epidermální růstový faktor MeSH
- erbB receptory MeSH
- folikuly stimulující hormon MeSH
- glukuronosyltransferasa MeSH
- isochinoliny MeSH
- kyselina hyaluronová MeSH
- molekuly buněčné adheze MeSH
- progesteron MeSH
- protein Smad2 MeSH
- protein Smad3 MeSH
- PTX3 protein MeSH Prohlížeč
- pyridiny MeSH
- pyrroly MeSH
- RTKI cpd MeSH Prohlížeč
- sérový amyloidový protein MeSH
- tyrphostiny MeSH
Several lines of evidence suggest that in mice the activation of SMAD2/3 signaling by oocyte secreted factors, together with epidermal growth factor receptor (EGFR) activation, is essential to induce cumulus expansion. Here we show that inhibition of EGFR kinase in follicle stimulating hormone (FSH)-stimulated porcine oocyte-cumulus cell complex (OCCs) strongly decreases hyaluronan (HA) synthesis and its retention in the matrix, as well as progesterone synthesis. Although porcine cumulus cells undergo expansion independently of oocytes, we use biochemical and gene expression analyses to show that they do require activation of SMAD2/3 for optimal stimulation of HA synthesis and proteins involved in the organization of this polymer in the expanded matrix. Furthermore, FSH-induced progesterone synthesis by porcine cumulus cells was increased by blocking SMAD2/3 activation. In conclusion, these results support the hypothesis that an FSH-EGF autocrine loop is active in porcine OCCs, and provide the first evidence that the SMAD2/3 signaling pathway is induced by paracrine/autocrine factors in porcine cumulus cells and is involved in the control of both cumulus expansion and steroidogenesis.
Citace poskytuje Crossref.org
The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction
