Molecular detection of trophic links in a complex insect host-parasitoid food web
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
- MeSH
- Diptera genetika MeSH
- DNA primery genetika MeSH
- druhová specificita MeSH
- fylogeneze MeSH
- Hymenoptera genetika MeSH
- interakce hostitele a parazita genetika MeSH
- larva parazitologie MeSH
- Lepidoptera genetika parazitologie MeSH
- molekulární sekvence - údaje MeSH
- potravní řetězec * MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- taxonomické DNA čárové kódování MeSH
- tropické klima MeSH
- výpočetní biologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Geografické názvy
- Papua Nová Guinea MeSH
- Názvy látek
- DNA primery MeSH
Previously, host-parasitoid links have been unveiled almost exclusively by time-intensive rearing, while molecular methods were used only in simple agricultural host-parasitoid systems in the form of species-specific primers. Here, we present a general method for the molecular detection of these links applied to a complex caterpillar-parasitoid food web from tropical rainforest of Papua New Guinea. We DNA barcoded hosts, parasitoids and their tissue remnants and matched the sequences to our extensive library of local species. We were thus able to match 87% of host sequences and 36% of parasitoid sequences to species and infer subfamily or family in almost all cases. Our analysis affirmed 93 hitherto unknown trophic links between 37 host species from a wide range of Lepidoptera families and 46 parasitoid species from Hymenoptera and Diptera by identifying DNA sequences for both the host and the parasitoid involved in the interaction. Molecular detection proved especially useful in cases where distinguishing host species in caterpillar stage was difficult morphologically, or when the caterpillar died during rearing. We have even detected a case of extreme parasitoid specialization in a pair of Choreutis species that do not differ in caterpillar morphology and ecology. Using the molecular approach outlined here leads to better understanding of parasitoid host specificity, opens new possibilities for rapid surveys of food web structure and allows inference of species associations not already anticipated.
Citace poskytuje Crossref.org
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