The midgut transcriptome of Phlebotomus (Larroussius) perniciosus, a vector of Leishmania infantum: comparison of sugar fed and blood fed sand flies
Jazyk angličtina Země Velká Británie, Anglie Médium electronic
Typ dokumentu srovnávací studie, časopisecké články, Research Support, N.I.H., Intramural, práce podpořená grantem
Grantová podpora
078937
Wellcome Trust - United Kingdom
Intramural NIH HHS - United States
PubMed
21569254
PubMed Central
PMC3107814
DOI
10.1186/1471-2164-12-223
PII: 1471-2164-12-223
Knihovny.cz E-zdroje
- MeSH
- databáze genetické MeSH
- fylogeneze MeSH
- genová knihovna MeSH
- hmyz - vektory klasifikace cytologie enzymologie genetika MeSH
- krev * MeSH
- Leishmania infantum * MeSH
- messenger RNA genetika metabolismus MeSH
- mikroklky genetika MeSH
- molekulární sekvence - údaje MeSH
- orgánová specificita MeSH
- oxidační stres genetika MeSH
- Phlebotomus klasifikace cytologie enzymologie genetika MeSH
- sacharidy * MeSH
- sekvence aminokyselin MeSH
- sekvenční analýza DNA MeSH
- stanovení celkové genové exprese * MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Intramural MeSH
- srovnávací studie MeSH
- Názvy látek
- messenger RNA MeSH
- sacharidy * MeSH
BACKGROUND: Parasite-vector interactions are fundamental in the transmission of vector-borne diseases such as leishmaniasis. Leishmania development in the vector sand fly is confined to the digestive tract, where sand fly midgut molecules interact with the parasites. In this work we sequenced and analyzed two midgut-specific cDNA libraries from sugar fed and blood fed female Phlebotomus perniciosus and compared the transcript expression profiles. RESULTS: A total of 4111 high quality sequences were obtained from the two libraries and assembled into 370 contigs and 1085 singletons. Molecules with putative roles in blood meal digestion, peritrophic matrix formation, immunity and response to oxidative stress were identified, including proteins that were not previously reported in sand flies. These molecules were evaluated relative to other published sand fly transcripts. Comparative analysis of the two libraries revealed transcripts differentially expressed in response to blood feeding. Molecules up regulated by blood feeding include a putative peritrophin (PperPer1), two chymotrypsin-like proteins (PperChym1 and PperChym2), a putative trypsin (PperTryp3) and four putative microvillar proteins (PperMVP1, 2, 4 and 5). Additionally, several transcripts were more abundant in the sugar fed midgut, such as two putative trypsins (PperTryp1 and PperTryp2), a chymotrypsin (PperChym3) and a microvillar protein (PperMVP3). We performed a detailed temporal expression profile analysis of the putative trypsin transcripts using qPCR and confirmed the expression of blood-induced and blood-repressed trypsins. Trypsin expression was measured in Leishmania infantum-infected and uninfected sand flies, which identified the L. infantum-induced down regulation of PperTryp3 at 24 hours post-blood meal. CONCLUSION: This midgut tissue-specific transcriptome provides insight into the molecules expressed in the midgut of P. perniciosus, an important vector of visceral leishmaniasis in the Old World. Through the comparative analysis of the libraries we identified molecules differentially expressed during blood meal digestion. Additionally, this study provides a detailed comparison to transcripts of other sand flies. Moreover, our analysis of putative trypsins demonstrated that L. infantum infection can reduce the transcript abundance of trypsin PperTryp3 in the midgut of P. perniciosus.
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