Transplantation of predifferentiated adipose-derived stromal cells for the treatment of spinal cord injury

. 2011 Oct ; 31 (7) : 1113-22. [epub] 20110601

Jazyk angličtina Země Nizozemsko Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid21630007

Adipose-derived stromal cells (ASCs) are an alternative source of stem cells for cell-based therapies of neurological disorders such as spinal cord injury (SCI). In the present study, we predifferentiated ASCs (pASCs) and compared their behavior with naïve ASCs in vitro and after transplantation into rats with a balloon-induced compression lesion. ASCs were predifferentiated into spheres before transplantation, then pASCs or ASCs were injected intraspinally 1 week after SCI. The cells' fate and the rats' functional outcome were assessed using behavioral, histological, and electrophysiological methods. Immunohistological analysis of pASCs in vitro revealed the expression of NCAM, NG2, S100, and p75. Quantitative RT-PCR at different intervals after neural induction showed the up-regulated expression of the glial markers NG2 and p75 and the neural precursor markers NCAM and Nestin. Patch clamp analysis of pASCs revealed three different types of membrane currents; however, none were fast activating Na(+) currents indicating a mature neuronal phenotype. Significant improvement in both the pASC and ASC transplanted groups was observed in the BBB motor test. In vivo, pASCs survived better than ASCs did and interacted closely with the host tissue, wrapping host axons and oligodendrocytes. Some transplanted cells were NG2- or CD31-positive, but no neuronal markers were detected. The predifferentiation of ASCs plays a beneficial role in SCI repair by promoting the protection of denuded axons; however, functional improvements were comparable in both the groups, indicating that repair was induced mainly through paracrine mechanisms.

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Abramson S (2010) New views of modern medicine regarding treatment with stem cells; its practical and ethical consequences. Klin Onkol 23(1):10–13 PubMed

Amemori T, Jendelová P, Růzicková K, Arboleda D, Syková E (2010) Co-transplantation of olfactory ensheathing glia and mesenchymal stromal cells does not have synergistic effects after spinal cord injury in the rat. Cytotherapy 12(2):212–225 PubMed

Bae JS, Carter JE, Jin HK (2010) Adipose tissue-derived stem cells rescue Purkinje neurons and alleviate inflammatory responses in Niemann-Pick disease type C mice. Cell Tissue Res 340(2):357–369 PubMed

Basso DM, Beattie MS, Bresnahan JC (1995) A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 12(1):1–21 PubMed

Chan J, Aoki C, Pickel VM (1990) Optimization of differential immunogold-silver and peroxidase labeling with maintenance of ultrastructure in brain sections before plastic embedding. J Neurosci Methods 33(2-3):113–127 PubMed PMC

Chi GF, Kim MR, Kim DW, Jiang MH, Son Y (2010) Schwann cells differentiated from spheroid-forming cells of rat subcutaneous fat tissue myelinate axons in the spinal cord injury. Exp Neurol 222(2):304–317 PubMed

Chiu SC, Hung HS, Lin SZ, Chiang E, Liu DD (2009) Therapeutic potential of olfactory ensheathing cells in neurodegenerative diseases. J Mol Med 87(12):1179–1189 PubMed

Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, Andriolo G, Sun B, Zheng B, Zhang L, Norotte C, Teng PN, Traas J, Schugar R, Deasy BM, Badylak S, Buhring HJ, Giacobino JP, Lazzari L, Huard J, Peault B (2008) A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3:301–313 PubMed

Fang Z, Yang Q, Xiong W, Li G, Xiao J, Guo F, Li F, Chen A (2010) Neurogenic differentiation of murine adipose derived stem cells transfected with EGFP in vitro. J Huazhong Univ Sci Technol Med Sci 30(1):75–80 PubMed

Franco Lambert AP, Fraga Zandonai A, Bonatto D, Cantarelli Machado D, Pêgas Henriques JA (2009) Differentiation of human adipose-derived adult stem cells into neuronal tissue: does it work? Differentiation 77(3):221–228 PubMed

Gordon D, Scolding NJ (2009) Human mesenchymal stem cell culture for neural transplantation. Methods Mol Biol 549:103–118 PubMed

Gu W, Zhang F, Xue Q, Ma Z, Lu P, Yu B (2010) Transplantation of bone marrow mesenchymal stem cells reduces lesion volume and induces axonal regrowth of injured spinal cord. Neuropathology 30(3):205–217 PubMed

Halliday GM, Cullen KM, Kril JJ, Harding AJ, Harasty J (1996) Glial fibrillary acidic protein (GFAP) immunohistochemistry in human cortex: a quantitative study using different antisera. Neurosci Lett 209(1):29–32 PubMed

Harris LJ, Zhang P, Abdollahi H, Tarola NA, DiMatteo C, McIlhenny SE, Tulenko TN, DiMuzio PJ (2010) Availability of adipose-derived stem cells in patients undergoing vascular surgical procedures. J Surg Res 163(2):e105–e112 PubMed PMC

Hejcl A, Sedý J, Kapcalová M, Toro DA, Amemori T, Lesný P, Likavcanová-Mašínová K, Krumbholcová E, Prádný M, Michálek J, Burian M, Hájek M, Jendelová P, Syková E (2010) HPMA-RGD hydrogels seeded with mesenchymal stem cells improve functional outcome in chronic spinal cord injury. Stem Cells Dev 19(10):1535–1546 PubMed

Hess PG (2009) Risk of tumorigenesis in first-in-human trials of embryonic stem cell neural derivatives: ethics in the face of long-term uncertainty. Account Res 16(4):175–198 PubMed

Hong SJ, Traktuev DO, March KL (2010) Therapeutic potential of adipose-derived stem cells in vascular growth and tissue repair. Curr Opin Organ Transplant 15(1):86–91 PubMed

Hu BY, Zhang SC (2010) Directed differentiation of neural-stem cells and subtype-specific neurons from hESCs. Methods Mol Biol 636:123–137 PubMed PMC

Karimi-Abdolrezaee S, Eftekharpour E, Wang J, Schut D, Fehlings MG (2010) Synergistic effects of transplanted adult neural stem/progenitor cells, chondroitinase, and growth factors promote functional repair and plasticity of the chronically injured spinal cord. J Neurosci 30(5):1657–1676 PubMed PMC

Khoo ML, Shen B, Tao H, Ma DD (2008) Long-term serial passage and neuronal differentiation capability of human bone marrow mesenchymal stem cells. Stem Cells Dev 17(5):883–896 PubMed

Knerlich-Lukoschus F, von der Ropp-Brenner B, Lucius R, Mehdorn HM, Held-Feindt J (2010) Chemokine, expression in the white matter spinal cord precursor niche after force-defined spinal cord contusion injuries in adult rats. Glia 58(8):916–931 PubMed

Lee ST, Chu K, Jung KH, Im WS, Park JE, Lim HC, Won CH, Shin SH, Lee SK, Kim M, Roh JK (2009) Slowed progression in models of Huntington disease by adipose stem cell transplantation. Ann Neurol 66(5):671–681 PubMed

Leranth C, Pickel VM (1989) Electron microscopic pre-embedding double immunostaining methods. In: Heimer L, Zaborsky L (eds) Tract-tracing, vol II. Plenum Publishing, New York, pp 129–172

Lo B, Parham L, Cedars M, Fisher S, Gates E, Giudice L, Halme DG, Hershon W, Kriegstein A, Rao R, Roberts C, Wagner R (2010) RESEARCH ETHICS: NIH guidelines for stem cell research and gamete donors. Science 327(5968):962–963 PubMed PMC

Mantovani C, Mahay D, Kingham M, Terenghi G, Shawcross SG, Wiberg M (2010) Bone marrow- and adipose-derived stem cells show expression of myelin mRNAs and proteins. Regen Med 5(3):403–410 PubMed

Mizuno H (2009) Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review. J Nippon Med Sch 76(2):56–66 PubMed

Nakagami H, Maeda K, Morishita R, Iguchi S, Nishikawa T, Takami Y, Kikuchi Y, Saito Y, Tamai K, Ogihara T, Kaneda Y (2005) Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue-derived stromal cells. Arterioscler Thromb Vasc Biol 25(12):2542–2547 PubMed

Neri M, Maderna C, Ferrari D, Cavazzin C, Vescovi AL, Gritti A (2010) Robust generation of oligodendrocyte progenitors from human neural stem cells and engraftment in experimental demyelination models in mice. PLoS One 5(4):e10145 PubMed PMC

Ohta Y, Takenaga M, Tokura Y, Hamaguchi A, Matsumoto T, Kano K, Mugishima H, Okano H, Igarashi (2008) Mature adipocyte-derived cells, dedifferentiated fat cells (DFAT), promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. Cell Transplant 17(8):877–886 PubMed

Park HW, Lim MJ, Jung H, Lee SP, Paik KS, Chang MS (2010) Human mesenchymal stem cell-derived Schwann cell-like cells exhibit neurotrophic effects, via distinct growth factor production, in a model of spinal cord injury. Glia 58(9):1118–1132 PubMed

Pivonkova H, Benesova J, Butenko O, Chvatal A, Anderova M (2010) Impact of global cerebral ischemia on K+ channel expression and membrane properties of glial cells in the rat hippocampus. Neurochem Int 57(7):783–794 PubMed

Radtke C, Schmitz B, Spies M, Kocsis JD, Vogt PM (2009) Peripheral glial cell differentiation from neurospheres derived from adipose mesenchymal stem cells. Int J Dev Neurosci 27(8):817–823 PubMed

Rodríguez JJ, Dallérac GM, Tabuchi M, Davies HA, Colyer FM, Stewart MG, Doyère V (2008) N-methyl-d-aspartate receptor independent changes in expression of polysialic acid-neural cell adhesion molecule despite blockade of homosynaptic long-term potentiation and heterosynaptic long-term depression in the awake freely behaving rat dentate gyrus. Neuron Glia Biol 4(3):169–178 PubMed

Salgado AJ, Reis RL, Sousa NJ, Gimble JM (2010) Adipose tissue derived stem cells secretome: soluble factors and their roles in regenerative medicine. Curr Stem Cell Res Ther 5(2):103–110 PubMed

Sofroniew MV, Howe CL, Mobley WC (2001) Nerve growth factor signaling, neuroprotection, and neural repair. Annu Rev Neurosci 24:1217–1281 PubMed

Syková E, Jendelová P, Urdzíková L, Lesný P, Hejcl A (2006) Bone marrow stem cells and polymer hydrogels—two strategies for spinal cord injury repair. Cell Mol Neurobiol 26(7–8):1113–1129 PubMed PMC

Turnovcova K, Ruzickova K, Vanecek V, Sykova E, Jendelova P (2009) Properties and growth of human bone marrow mesenchymal stromal cells cultivated in different media. Cytotherapy 11(7):874–885 PubMed

Urdzikova L, Jendelova P, Glogarova K, Burian M, Hajek M, Sykova E (2006) Transplantation of bone marrow stem cells as well as mobilization by granulocyte-colony stimulating factor promotes recovery after spinal cord injury in rats. J Neurotrauma 23:1379–1391 PubMed

Wei X, Zhao L, Zhong J, Gu H, Feng D, Johnstone BH, March KL, Farlow MR, Du Y (2009) Adipose stromal cells-secreted neuroprotective media against neuronal apoptosis. Neurosci Lett 462(1):76–79 PubMed

Weishaupt N, Silasi G, Colbourne F, Fouad K (2010) Secondary damage in the spinal cord following motor cortex injury in rats. J Neurotrauma 27(8):1387–1397 PubMed

Xu Y, Liu Z, Liu L, Zhao C, Xiong F, Zhou C, Li Y, Shan Y, Peng F, Zhang C (2008) Neurospheres from rat adipose-derived stem cells could be induced into functional Schwann cell-like cells in vitro. BMC Neurosci 9:21 PubMed PMC

Yamada T, Akamatsu H, Hasegawa S, Yamamoto N, Yoshimura T, Hasebe Y, Inoue Y, Mizutani H, Uzawa T, Matsunaga K, Nakata S (2010) Age-related changes of p75 neurotrophin receptor-positive adipose-derived stem cells. J Dermatol Sci 58(1):36–42 PubMed

Zhang HT, Luo J, Sui LS, Ma X, Yan ZJ, Lin JH, Wang YS, Chen YZ, Jiang XD, Xu RX (2009) Effect of differentiated versus undifferentiated adipose tissue-derived stromal cell grafts on functional recovery after spinal cord contusion. Cell Mol Neurobiol 29:1283–1292 PubMed PMC

Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7(2):211–228 PubMed

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