Rapid monitoring of mono- and disaccharides in drinks, foodstuffs and foodstuff additives by capillary electrophoresis with contactless conductivity detection
Language English Country Netherlands Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21645652
DOI
10.1016/j.aca.2011.04.055
PII: S0003-2670(11)00610-6
Knihovny.cz E-resources
- MeSH
- Food Analysis instrumentation methods MeSH
- Time Factors MeSH
- Disaccharides analysis MeSH
- Electric Conductivity MeSH
- Electrophoresis, Capillary instrumentation methods MeSH
- Limit of Detection MeSH
- Monosaccharides analysis MeSH
- Beverages analysis MeSH
- Food Additives analysis MeSH
- Ribose analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Disaccharides MeSH
- Monosaccharides MeSH
- Food Additives MeSH
- Ribose MeSH
A capillary electrophoresis (CE) procedure with contactless conductivity detection (C(4)D) has been developed for monitoring of neutral mono- and disaccharides in drinks and foodstuffs. The separation of a mixture of seven neutral saccharides (glucose, fructose, galactose, mannose, ribose, sucrose and lactose) employed a quartz capillary, 5 μm i.d., with an effective length of 18.3 cm, and 75 mM NaOH (pH 12.8) as the background electrolyte (BGE). The limit of detection (LOD) values obtained lied within a range from 0.4 μmol L(-1) for lactose to 0.9 μmol L(-1) for ribose, with a separation time shorter than 140 s. The procedure was successfully applied to determinations of saccharides in fruit juices, Coca-Cola, milk, red and white wines, yoghurts, honey and a foodstuff additive.
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