Molecular imaging of brain lipid environment of lymphocytes in amyotrophic lateral sclerosis using magnetic resonance imaging and SECARS microscopy
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21777015
DOI
10.33549/physiolres.932187
PII: 932187
Knihovny.cz E-resources
- MeSH
- Amyotrophic Lateral Sclerosis genetics immunology metabolism pathology MeSH
- CD4-Positive T-Lymphocytes immunology metabolism pathology MeSH
- Dextrans MeSH
- Contrast Media MeSH
- Rats MeSH
- Humans MeSH
- Magnetic Resonance Imaging * MeSH
- Magnetite Nanoparticles MeSH
- Lipid Metabolism * MeSH
- Microscopy * MeSH
- Disease Models, Animal MeSH
- Molecular Imaging methods MeSH
- Brain immunology metabolism pathology MeSH
- Mutation MeSH
- Lipid Peroxidation MeSH
- Rats, Sprague-Dawley MeSH
- Rats, Transgenic MeSH
- Antibodies MeSH
- Spectrum Analysis, Raman * MeSH
- Superoxide Dismutase-1 MeSH
- Superoxide Dismutase genetics MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dextrans MeSH
- ferumoxtran-10 MeSH Browser
- Contrast Media MeSH
- Magnetite Nanoparticles MeSH
- Antibodies MeSH
- SOD1 protein, human MeSH Browser
- Sod1 protein, rat MeSH Browser
- Superoxide Dismutase-1 MeSH
- Superoxide Dismutase MeSH
This paper highlights some of the key technologies of using two innovative molecular imaging modalites, magnetic resonance imaging (MRI) and nonlinear optical microscopy, for imaging intravenously injected ultra small paramagnetic iron oxide nanoparticles cross linked with antibodies (CLUSPIO) in the amyotrophic lateral sclerosis (ALS) experimental model in vivo or ex vivo, respectively. Intensive efforts have been made in investigating the causes of abnormalities in lipid metabolism, monitored in some neurodegenerative disorders systems. It has been shown that an abnormal accumulation of some common lipids in motor nerve cells may play a critical role in the development of amyotrophic lateral sclerosis. The presented experiments were performed on brain specimens from the transgenic rat model expressing multiple copies of mutated (G93A) human SOD-1 gene, after CD4+ lymphocytes were magnetically labeled with i.v.i. CLUSPIO antibodies. In vivo MRI revealed marked signal intensity enhancements in specific pathological regions of the ALS rat brain as compared to the wild type. Surface-enhanced coherent anti-Stokes Raman scattering (SECARS) microscopy indicated cellular interactions based on lipids association to anti-CD4 CLUSPIO.
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