Poly(ethylene oxide) layers grafted to dopamine-melanin anchoring layer: stability and resistance to protein adsorption
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21823677
DOI
10.1021/bm2007086
Knihovny.cz E-resources
- MeSH
- Adsorption MeSH
- Coated Materials, Biocompatible analysis chemical synthesis MeSH
- Biosensing Techniques methods MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Blood Proteins chemistry metabolism MeSH
- Humans MeSH
- Melanins chemistry MeSH
- Microscopy, Atomic Force MeSH
- Polyethylene Glycols chemistry MeSH
- Surface Properties MeSH
- Cattle MeSH
- Tissue Engineering methods MeSH
- Microscopy, Electron, Transmission MeSH
- Protein Binding MeSH
- Chromatography, High Pressure Liquid MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Coated Materials, Biocompatible MeSH
- dopamine melanin MeSH Browser
- Blood Proteins MeSH
- Melanins MeSH
- Polyethylene Glycols MeSH
In this study, we propose substrate-independent modification for creating a protein-repellent surface based on dopamine-melanin anchoring layer used for subsequent binding of poly(ethylene oxide) (PEO) from melt. We verified that the dopamine-melanin layer can be formed on literally any substrate and could serve as the anchoring layer for subsequent grafting of PEO chains. Grafting of PEO from melt in a temperature range 70-110 °C produces densely packed PEO layers showing exceptionally low protein adsorption when exposed to the whole blood serum or plasma. The PEO layers prepared from melt at 110 °C retained the protein repellent properties for as long as 10 days after their exposure to physiological-like conditions. The PEO-dopamine-melanin modification represents a simple and universal surface modification method for the preparation of protein repellent surfaces that could serve as a nonfouling background in various applications, such as optical biosensors and tissue engineering.
References provided by Crossref.org
Nonfouling poly(ethylene oxide) layers end-tethered to polydopamine
