The structure of the H107R variant of the extracellular domain of the mouse natural killer cell receptor NKR-P1A has been determined by X-ray diffraction at 2.3 Å resolution from a merohedrally twinned crystal. Unlike the structure of the wild-type receptor in space group I4(1)22 with a single chain per asymmetric unit, the crystals of the variant belonged to space group I4(1) with a dimer in the asymmetric unit. Different degrees of merohedral twinning were detected in five data sets collected from different crystals. The mutation does not have a significant impact on the overall structure, but led to the binding of an additional phosphate ion at the interface of the molecules.

Institute of Macromolecular Chemistry AS CR vvi Prague Czech Republic

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Aldemir, H., Prod’homme, V., Dumaurier, M.-J., Retiere, C., Poupon, G., Cazareth, J., Bihl, F. & Braud, V. M. (2005). J. Immunol. 175, 7791–7795. PubMed

Berman, H. M., Westbrook, J., Feng, Z., Gilliland, G., Bhat, T. N., Weissig, H., Shindyalov, I. N. & Bourne, P. E. (2000). Nucleic Acids Res. 28, 235–242. PubMed PMC

Bezouška, K., Yuen, C.-T., O’Brien, J., Childs, R. A., Chai, W., Lawson, A. M., Drbal, K., Fišerová, A., Pospíšil, M. & Feizi, T. (1994). Nature (London), 372, 150–157. PubMed

Carlyle, J. R., Jamieson, A. M., Gasser, S., Clingan, C. S., Arase, H. & Raulet, D. H. (2004). Proc. Natl Acad. Sci. USA, 101, 3527–3532. PubMed PMC

Chen, V. B., Arendall, W. B., Headd, J. J., Keedy, D. A., Immormino, R. M., Kapral, G. J., Murray, L. W., Richardson, J. S. & Richardson, D. C. (2010). Acta Cryst. D66, 12–21. PubMed PMC

Emsley, P. & Cowtan, K. (2004). Acta Cryst. D60, 2126–2132. PubMed

Iizuka, K., Naidenko, O. V., Plougastel, B. F., Fremont, D. H. & Yokoyama, W. M. (2003). Nature Immunol. 4, 801–807. PubMed

Kabsch, W. (2010). Acta Cryst. D66, 125–132. PubMed PMC

Kolenko, P., Rozbeský, D., Vaněk, O., Kopecký, V., Hofbauerová, K., Novák, P., Pompach, P., Hašek, J., Skálová, T., Bezouška, K. & Dohnálek, J. (2011). J. Struct. Biol. 175, 434–441. PubMed

Krissinel, E. & Henrick, K. (2007). J. Mol. Biol. 372, 774–797. PubMed

Kveberg, L., Dai, K. Z., Westgaard, I. H., Daws, M. R., Fossum, S., Naper, C. & Vaage, J. T. (2009). Eur. J. Immunol. 39, 541–551. PubMed

Makrigiannis, A. P., Pau, A. T., Saleh, A., Winkler-Pickett, R., Ortaldo, J. R. & Anderson, S. K. (2001). J. Immunol. 166, 5034–5043. PubMed

Mesci, A., Ljutic, B., Makrigiannis, A. P. & Carlyle, J. R. (2006). Immunol. Res. 35, 13–26. PubMed

Murshudov, G. N., Skubák, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M. D., Long, F. & Vagin, A. A. (2011). Acta Cryst. D67, 355–367. PubMed PMC

Rosen, D. B., Bettadapura, J., Alsharifi, M., Mathew, P. A., Warren, H. S. & Lanier, L. L. (2005). J. Immunol. 176, 7796–7799. PubMed

Skálová, T., Dušková, J., Hašek, J., Kolenko, P., Štěpánková, A. & Dohnálek, J. (2010). J. Appl. Cryst. 43, 737–742.

Sovová, Z., Kopecký, V., Pazderka, T., Hofbauerová, K., Rozbeský, D., Vaněk, O., Bezouška, K. & Ettrich, R. (2011). J. Mol. Model. 17, 1353–1370. PubMed

Vagin, A. & Teplyakov, A. (2010). Acta Cryst. D66, 22–25. PubMed

Vivier, E., Tomasello, E., Baratin, M., Walzer, T. & Ugolini, S. (2008). Nature Immunol. 9, 503–510. PubMed

Winn, M. D. et al. (2011). Acta Cryst. D67, 235–242. PubMed

Four crystal structures of human LLT1, a ligand of human NKR-P1, in varied glycosylation and oligomerization states

Nkrp1 family, from lectins to protein interacting molecules

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PDB
3T3A