Diversity, evolution and molecular systematics of the Psalteriomonadidae, the main lineage of anaerobic/microaerophilic heteroloboseans (excavata: discoba)
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22192530
DOI
10.1016/j.protis.2011.11.002
PII: S1434-4610(11)00120-9
Knihovny.cz E-resources
- MeSH
- Eukaryota classification cytology genetics MeSH
- Phylogeny MeSH
- Genes, rRNA MeSH
- Water Microbiology * MeSH
- Microscopy MeSH
- Molecular Sequence Data MeSH
- DNA, Protozoan chemistry genetics MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Protozoan genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Protozoan MeSH
- DNA, Ribosomal MeSH
- RNA, Protozoan MeSH
- RNA, Ribosomal, 18S MeSH
We isolated and cultivated 31 strains of free-living heterolobosean flagellates and amoebae from freshwater, brackish, and marine sediments with low concentrations of oxygen. Phylogenetic analysis of small subunit (SSU) rDNA showed that the strains constitute a single clade, the Psalteriomonadidae. According to combined light-microscopic morphology plus molecular phylogeny, our isolates belong to seven species and five genera, from which three species and two genera are new. In addition, previously described anaerobic species Percolomonas descissus and Vahlkampfia anaerobica are transferred to the Psalteriomonadidae. We identified a flagellate stage of Monopylocystis visvesvarai which was reported to produce only amoebae. Two environmental sequences previously obtained from acidic environments belong to the Psalteriomonadidae as well, suggesting a broad ecological importance of the Psalteriomonadidae. The ultrastructure of two psalteriomonadid species was also studied. Unifying features of the Psalteriomonadidae are acristate mitochondrial derivates, flagellates with a ventral groove and four flagella, and a harp-like structure in the mastigont. A new overall classification of the Psalteriomonadidae is proposed. Our data show that the Psalteriomonadidae are much more diverse than previously thought and constitute the main anaerobic lineage within the Heterolobosea.
References provided by Crossref.org
EukRef-excavates: seven curated SSU ribosomal RNA gene databases
Fe-S cluster assembly in the supergroup Excavata
Arginine deiminase pathway enzymes: evolutionary history in metamonads and other eukaryotes
GENBANK
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