The impact of the microbial load of genital mycoplasmas and gestational age on the intensity of intraamniotic inflammation
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22340945
DOI
10.1016/j.ajog.2012.01.004
PII: S0002-9378(12)00005-1
Knihovny.cz E-resources
- MeSH
- Amniocentesis MeSH
- Bacterial Load MeSH
- Chorioamnionitis diagnosis microbiology MeSH
- DNA, Bacterial analysis MeSH
- Adult MeSH
- Gestational Age * MeSH
- Pregnancy Complications, Infectious diagnosis microbiology MeSH
- Humans MeSH
- Inflammation Mediators analysis MeSH
- Young Adult MeSH
- Mycoplasma Infections diagnosis MeSH
- Amniotic Fluid chemistry microbiology MeSH
- Fetal Membranes, Premature Rupture microbiology MeSH
- Prospective Studies MeSH
- Severity of Illness Index MeSH
- Pregnancy MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Inflammation Mediators MeSH
OBJECTIVE: The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators. STUDY DESIGN: A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 24(0/7) and 36(6/7) weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology. RESULTS: Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used. CONCLUSION: The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age.
References provided by Crossref.org
Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes
Late preterm prelabor rupture of fetal membranes: fetal inflammatory response and neonatal outcome
Maternal Serum C-Reactive Protein in Women with Preterm Prelabor Rupture of Membranes
Intraamniotic Inflammation in Women with Preterm Prelabor Rupture of Membranes
Cervical microbiota in women with preterm prelabor rupture of membranes
