OBJECTIVE: The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators. STUDY DESIGN: A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 24(0/7) and 36(6/7) weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology. RESULTS: Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used. CONCLUSION: The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age.

Department of Obstetrics and Gynecology Charles University Prague Prague Czech Republic

Citace poskytuje Crossref.org

Presence of Chlamydia trachomatis DNA in the amniotic fluid in women with preterm prelabor rupture of membranes

Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes

Late preterm prelabor rupture of fetal membranes: fetal inflammatory response and neonatal outcome

Maternal Serum C-Reactive Protein in Women with Preterm Prelabor Rupture of Membranes

Intraamniotic Inflammation in Women with Preterm Prelabor Rupture of Membranes

Cervical microbiota in women with preterm prelabor rupture of membranes