Ureaplasma spp., commonly identified in the vagina/cervix of pregnant women with spontaneous preterm delivery, are the most frequently detected microorganisms in amniotic fluid. To date, 14 U. spp. serotypes have been characterized; however, modern molecular biology methods can distinguish different U. spp genotypes. Considering these factors, a knowledge gap exists regarding the association between U. spp. genotypes and the risk of the ascension of U. spp. from the cervix to the amniotic cavity. To fill this gap, an expanded multilocus sequence-typing scheme of U. spp. was performed to assess the relationship between cervical and amniotic fluid U. spp. in pregnant women with spontaneous preterm delivery. This study included 109 and 69 pregnant women with spontaneous preterm labor (PTL) and preterm prelabor rupture of membranes (PPROM), respectively. U. spp. DNA in cervical fluid was identified in 49% and 55% of the women with PTL and PPROM, respectively. The concurrent presence of U. spp. DNA in amniotic fluid was observed in 17% and 59% of the pregnant women with PTL and PPROM, respectively. Among pregnant women with PTL and PPROM, 38 expanded sequence types of cervical U. spp. were identified. No associations were observed between specific genotypes, subgroups, or clusters of cervical U. spp. and the presence of amniotic fluid U. spp. in pregnant women with spontaneous preterm delivery.

Biomedical Research Center University Hospital Hradec Kralove Hradec Kralove Czech Republic

Department of Genetics and Bioinformatics Domain of Health Data and Digitalisation Institute of Public Health Oslo Norway

Department of Gynecology and Obsetrics Medical University of Lodz Lodz Poland

Department of Non Medical Studies Faculty of Medicine in Hradec Kralove Charles University Hradec Kralove Czech Republic

Department of Obstetrics and Gynecology Hospital Most Krajska zdravotni a s Most Czech Republic

Department of Obstetrics and Gynecology Region Västra Götaland Sahlgrenska University Hospital Gothenburg Sweden

Department of Obstetrics and Gynecology University Hospital Olomouc Zdravotniku 248 7 Olomouc Czech Republic

Department of Perinatology Obstetrics and Gynecology Institute of Clinical Science Sahlgrenska Academy University of Gothenburg Gothenburg Sweden

Department of Perinatology Obstetrics and Gynecology Polish Mother's Memorial Hospital Lodz Poland

Generi Biotech Hradec Kralove Czech Republic

Institute of Clinical Microbiology Faculty of Medicine in Hradec Kralove Charles University Hradec Kralove Czech Republic

Institute of Clinical Microbiology University Hospital Hradec Kralove Czech Republic

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