To determine the IgGFc-binding protein (FcgammaBP) concentration in amniotic and cervical fluids in preterm prelabor rupture of membranes (PPROM) and preterm labor with intact membranes (PTL) and to assess the diagnostic indices of FcgammaBP to predict intra-amniotic infection (the presence of both microbial invasion of the amniotic cavity and intra-amniotic inflammation). In this study, we included 170 and 79 women with PPROM and PTL, respectively. Paired cervical and amniotic fluid samples were obtained using a Dacron polyester swab and transabdominal amniocentesis, respectively. The FcgammaBP concentrations in the samples were assessed using an enzyme-linked immunosorbent assay. The presence of intra-amniotic infection was associated with elevated FcgammaBP concentrations in pregnancies with PPROM and PTL [PPROM-presence: 86 ng/mL vs. absence: 13 ng/mL, p < 0.0001, area under receiver operating characteristic curve (AUC) = 0.94; PTL-presence: 140 ng/mL vs. absence: 22 ng/mL, p < 0.0001, AUC = 0.86]. In cervical fluid, the concentrations of FcgammaBP were elevated in the presence of intra-amniotic infection in pregnancies with PPROM only (presence: 345 ng/mL vs. absence: 60 ng/mL, p < 0.0001, AUC = 0.93). FcgammaBP in amniotic fluid might be a marker of intra-amniotic infection in women with both PPROM and PTL However, in cervical fluid, it is only observed in women with PPROM.

Biomedical Research Center University Hospital Hradec Kralove Hradec Králové Czech Republic

Department of Clinical Immunology and Allergy University Hospital Hradec Kralove Charles University Faculty of Medicine in Hradec Kralove Hradec Králové Czech Republic

Department of Genetics and Bioinformatics Domain of Health Data and Digitalisation Institute of Public Health Oslo Norway

Department of Obstetrics and Gynecology Institute of Clinical Science Sahlgrenska Academy University of Gothenburg Gothenburg Sweden

Department of Obstetrics and Gynecology Region Västra Götaland Sahlgrenska University Hospital Gothenburg Sweden

Department of Social Medicine Charles University Faculty of Medicine in Hradec Kralove Hradec Králové Czech Republic

Institute of Clinical Biochemistry and Diagnostics University Hospital Hradec Kralove Charles University Faculty of Medicine in Hradec Kralove Hradec Králové Czech Republic

Institute of Clinical Microbiology University Hospital in Hradec Kralove Charles University Faculty of Medicine Hradec Kralove Hradec Králové Czech Republic

The Department of Obstetrics and Gynecology University Hospital Hradec Kralove Charles University Faculty of Medicine in Hradec Kralove Sokolska 581 500 05 Hradec Králové Czech Republic

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