Expression profiles of fetal membrane nicotinamide adenine dinucleotide phosphate oxidases (NOX) 2 and 3 differentiates spontaneous preterm birth and pPROM pathophysiologies
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24439294
DOI
10.1016/j.placenta.2013.12.012
PII: S0143-4004(13)00872-2
Knihovny.cz E-zdroje
- Klíčová slova
- Cigarette smoking extract, Oxidase enzymes, Preterm birth, Preterm premature rupture of membranes, Reactive species of oxygen,
- MeSH
- dospělí MeSH
- extraembryonální obaly enzymologie MeSH
- kouření patofyziologie MeSH
- lidé MeSH
- membránové glykoproteiny biosyntéza MeSH
- membránové proteiny biosyntéza MeSH
- NADPH-oxidasa 2 MeSH
- NADPH-oxidasy biosyntéza MeSH
- novorozenec MeSH
- předčasný odtok plodové vody enzymologie MeSH
- předčasný porod enzymologie MeSH
- reaktivní formy kyslíku metabolismus MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- novorozenec MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CYBB protein, human MeSH Prohlížeč
- membránové glykoproteiny MeSH
- membránové proteiny MeSH
- NADPH-oxidasa 2 MeSH
- NADPH-oxidasy MeSH
- Nox3 protein, human MeSH Prohlížeč
- reaktivní formy kyslíku MeSH
INTRODUCTION: Nicotinamide adenine dinucleotide phosphate oxidases (NOX 1-5) are enzymes that generate cellular reactive oxygen species (ROS) besides mitochondria and might be important ROS sources associated with pregnancy complications, particularly preterm premature rupture of membranes (pPROM), that has been related to ROS. OBJECTIVE: To characterize NOX enzymes expression in human fetal membranes. METHODS: Differential expression and localization of NOX isoforms in human fetal membranes collected from women with uncomplicated pregnancies at term, preterm birth (PTB) or pPROM and in vitro in normal term membranes maintained in an organ explant system stimulated with water-soluble cigarette smoke extract (wsCSE) were documented by real time PCR and immunohistochemistry. RESULTS: Fetal membranes from term deliveries, PTB and pPROM expressed NOX 2, 3 and 4 mRNAs whereas NOX 1 and 5 were not detected. NOX 2 expression was 2.3-fold higher in PTB than pPROM (p = 0.005) whereas NOX 3 was 2.2-fold higher in pPROM compared to PTB (p = 0.04). NOX 2 and 3 expressions at term mimicked pPROM and PTB, respectively. No difference in NOX 4 expression was observed among the studied groups. NOX 2, 3 and 4 were localized to both amniotic and chorionic cells. Expression of NOX 2, 3 and 4 were not significant in wsCSE-stimulated membranes compared to untreated controls. DISCUSSION/CONCLUSIONS: NOX enzymes are present in the fetal membranes and are differentially expressed in PTB and pPROM. Absence of any changes in NOXs expression after wsCSE stimulation suggests ROS generation in the membranes does not always correlate with NOX expression.
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