Bovine serum albumin film as a template for controlled nanopancake and nanobubble formation: in situ atomic force microscopy and nanolithography study
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22341519
DOI
10.1016/j.colsurfb.2012.01.028
PII: S0927-7765(12)00053-7
Knihovny.cz E-resources
- MeSH
- Ethanol MeSH
- Graphite chemistry MeSH
- Microscopy, Atomic Force MeSH
- Nanostructures chemistry ultrastructure MeSH
- Nanotechnology MeSH
- Gases MeSH
- Surface Properties MeSH
- Serum Albumin, Bovine chemistry MeSH
- Cattle MeSH
- Water MeSH
- Air MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Ethanol MeSH
- Graphite MeSH
- Gases MeSH
- Serum Albumin, Bovine MeSH
- Water MeSH
Air nanobubbles and nanopancakes were investigated in situ by both tapping mode atomic force microscopy (TM AFM) and atomic force nanolithography techniques employing bovine serum albumin (BSA) film supported by highly oriented pyrolytic graphite (HOPG). The BSA denaturation induced by the water-to-ethanol exchange served for conservation of nanobubble and nanopancake sites appearing as imprints in BSA film left by gaseous cavities formerly present on the interface in the aqueous environment. Once the BSA film was gently removed by the nanoshaving technique applied in ethanol, a clean basal plane HOPG area with well-defined dimensions was regenerated. The subsequent reverse ethanol-to-water exchange led to the re-formation of nanopancakes specifically at the nanoshaved area. Our approach paves the way for the study of gaseous nanostructures with defined dimensions, formed at solid-liquid interface under controlled conditions.
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