Bovine serum albumin film as a template for controlled nanopancake and nanobubble formation: in situ atomic force microscopy and nanolithography study
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22341519
DOI
10.1016/j.colsurfb.2012.01.028
PII: S0927-7765(12)00053-7
Knihovny.cz E-zdroje
- MeSH
- ethanol MeSH
- grafit chemie MeSH
- mikroskopie atomárních sil MeSH
- nanostruktury chemie ultrastruktura MeSH
- nanotechnologie MeSH
- plyny MeSH
- povrchové vlastnosti MeSH
- sérový albumin hovězí chemie MeSH
- skot MeSH
- voda MeSH
- vzduch MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ethanol MeSH
- grafit MeSH
- plyny MeSH
- sérový albumin hovězí MeSH
- voda MeSH
Air nanobubbles and nanopancakes were investigated in situ by both tapping mode atomic force microscopy (TM AFM) and atomic force nanolithography techniques employing bovine serum albumin (BSA) film supported by highly oriented pyrolytic graphite (HOPG). The BSA denaturation induced by the water-to-ethanol exchange served for conservation of nanobubble and nanopancake sites appearing as imprints in BSA film left by gaseous cavities formerly present on the interface in the aqueous environment. Once the BSA film was gently removed by the nanoshaving technique applied in ethanol, a clean basal plane HOPG area with well-defined dimensions was regenerated. The subsequent reverse ethanol-to-water exchange led to the re-formation of nanopancakes specifically at the nanoshaved area. Our approach paves the way for the study of gaseous nanostructures with defined dimensions, formed at solid-liquid interface under controlled conditions.
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