Improvac does not modify the expression and activities of the major drug metabolizing enzymes cytochrome P450 3A and 2C in pigs
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22484351
DOI
10.1016/j.vaccine.2012.03.072
PII: S0264-410X(12)00464-1
Knihovny.cz E-resources
- MeSH
- Vaccines, Contraceptive administration & dosage adverse effects MeSH
- Quinolines metabolism MeSH
- Cytochrome P-450 CYP3A biosynthesis MeSH
- Gene Expression drug effects MeSH
- Liver enzymology MeSH
- Castration adverse effects MeSH
- Constitutive Androstane Receptor MeSH
- Coumarins metabolism MeSH
- Swine MeSH
- Pregnane X Receptor MeSH
- Receptors, Cytoplasmic and Nuclear biosynthesis MeSH
- Receptors, Steroid biosynthesis MeSH
- Cytochrome P-450 Enzyme System biosynthesis MeSH
- Tolbutamide metabolism MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 7-benzyloxy-4-trifluoromethylcoumarin MeSH Browser
- 7-benzyloxyquinoline MeSH Browser
- Vaccines, Contraceptive MeSH
- Quinolines MeSH
- Cytochrome P-450 CYP3A MeSH
- cytochrome P-450 CYP2C subfamily MeSH Browser
- Constitutive Androstane Receptor MeSH
- Coumarins MeSH
- Pregnane X Receptor MeSH
- Receptors, Cytoplasmic and Nuclear MeSH
- Receptors, Steroid MeSH
- Cytochrome P-450 Enzyme System MeSH
- Tolbutamide MeSH
In the present study, we investigated hepatic mRNA expression and activities of CYP3A and 2C in entire, surgically castrated and pigs vaccinated with Improvac. Additionally, we examined the mRNA expression of the two nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR), known to regulate CYP3A and 2C mRNA expression, respectively. Activities of CYP3A and 2C were estimated as a rate of 7-benzyloxy-4-trifluoromethylcoumarin and 7-benzyloxyquinoline metabolism (CYP3A) and tolbutamide metabolism (CYP2C). We found no effect of Improvac treatment or surgical castration on either CYP3A or 2C activities. Similarly, the mRNA expressions of CYP3A29, 2C33 and PXR were not changed. CAR mRNA expression differed only between entire and surgically castrated male pigs (p=0.005), being greater in surgically castrated pigs. Our results indicated that neither CYP3A nor 2C are affected by Improvac.
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