Genetic diversity analysis and development of SCAR marker for detection of Indian populations of Fusarium oxysporum f. sp. ciceris causing chickpea wilt
Language English Country United States Media print-electronic
Document type Journal Article
- MeSH
- Cicer microbiology MeSH
- DNA, Fungal chemistry genetics MeSH
- DNA Primers genetics MeSH
- Fusarium classification genetics isolation & purification MeSH
- Genetic Variation * MeSH
- Genotype MeSH
- Molecular Typing MeSH
- Mycological Typing Techniques MeSH
- Plant Diseases microbiology MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Random Amplified Polymorphic DNA Technique MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- India MeSH
- Names of Substances
- DNA, Fungal MeSH
- DNA Primers MeSH
Genetic diversity of the isolates of Fusarium oxysporum f. sp. ciceris causing chickpea wilt collected from 12 states representing different agro-ecological regions of India was determined through randomly amplified polymorphic DNA (RAPD) markers. The UPGMA cluster analysis grouped the isolates into eight categories showing high magnitude of genetic diversity. Each group had the isolates from different states present in various agro-ecological regions of India. Therefore, the groups generated through the RAPD analysis were not corresponding to area of the origin of the isolates. The RAPD primers, namely, OPA 7 and OPA 11 produced Foc specific fragment of ≈1.3 kb and ≈1.4 kb, respectively in all the isolates. These fragments were eluted, purified, cloned in pGEM-T Easy vector and sequenced. Primers were designed with sequence information of these two fragments using primer.3 software. Two sets of sequence characterized amplified region markers (SC-FOC 1 and SC-FOC 2) developed from the sequences of these fragments were found to be specific to Foc and produced an amplicon of 1.3 and 1.4 kb, respectively. These set of markers were validated against the isolates of the pathogen collected from different locations of India representing various races of the pathogen. They are non-specific to the other Fusarium species, Rhizoctonia solani and R. bataticola.
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