Strategies are sought to reduce intestinal colonisation of food-producing animals by Campylobacter jejuni, a leading bacterial cause of human foodborne illness worldwide. Presently, we tested the antimicrobial activity of hydrolysable-rich blackberry, cranberry and chestnut tannin extracts and condensed tannin-rich mimosa, quebracho and sorghum tannins (each at 100 mg/mL) against C. jejuni via disc diffusion assay in the presence of supplemental casamino acids. We found that when compared to non-tannin-treated controls, all tested tannins inhibited the growth of C. jejuni and that inhibition by the condensed tannin-rich mimosa and quebracho extracts was mitigated in nutrient-limited medium supplemented with casamino acids. When tested in broth culture, both chestnut and mimosa extracts inhibited growth of C. jejuni and this inhibition was much greater in nutrient-limited than in full-strength medium. Consistent with observations from the disc diffusion assay, the inhibitory activity of the condensed tannin-rich mimosa extracts but not the hydrolysable tannin-rich chestnut extracts was mitigated by casamino acid supplementation to the nutrient-limited medium, likely because the added amino acids saturated the binding potential of the condensed tannins. These results demonstrate the antimicrobial activity of various hydrolysable and condensed tannin-rich extracts against C. jejuni and reveal that condensed tannins may be less efficient than hydrolysable tannins in controlling C. jejuni in gut environments containing high concentrations of amino acids and soluble proteins.

United States Department of Agriculture Agricultural Research Service Southern Plains Agricultural Research Center College Station TX 77845 USA

Zobrazit více v PubMed

Int J Food Microbiol. 2010 Apr 30;139(1-2):102-7 PubMed

Arch Biochem Biophys. 1994 Jan;308(1):278-84 PubMed

Anaerobe. 2009 Feb-Apr;15(1-2):18-25 PubMed

Food Chem Toxicol. 1998 Dec;36(12):1053-60 PubMed

Oecologia. 1987 Apr;72(1):148-156 PubMed

J Appl Microbiol. 2009 Oct;107(4):1258-68 PubMed

Appl Environ Microbiol. 2011 Jan;77(1):57-66 PubMed

J Environ Sci Health B. 2011;46(4):360-5 PubMed

Biosci Biotechnol Biochem. 1999 Dec;63(12):2252-5 PubMed

Euro Surveill. 2007 Dec 20;12(12):E071220.6 PubMed

J Antimicrob Chemother. 2002 Dec;50(6):889-93 PubMed

Foodborne Pathog Dis. 2008 Jun;5(3):329-37 PubMed

J Agric Food Chem. 2006 Dec 13;54(25):9329-39 PubMed

Antonie Van Leeuwenhoek. 2009 Nov;96(4):377-94 PubMed

Vet Parasitol. 2001 Aug 20;99(3):205-19 PubMed

Mol Nutr Food Res. 2009 Sep;53 Suppl 2:S310-29 PubMed

Nutr Cancer. 2006;54(1):18-32 PubMed

Phytochemistry. 2004 May;65(9):1199-221 PubMed

Microbiology (Reading). 2002 Mar;148(Pt 3):685-694 PubMed

J AOAC Int. 2005 May-Jun;88(3):967-87 PubMed