Novosphingobium barchaimii sp. nov., isolated from hexachlorocyclohexane-contaminated soil
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny * MeSH
- Hexachlorocyclohexane * MeSH
- Nucleic Acid Hybridization MeSH
- Soil Pollutants MeSH
- Fatty Acids analysis MeSH
- Molecular Sequence Data MeSH
- Soil Microbiology * MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Spermidine analysis MeSH
- Sphingomonadaceae classification genetics isolation & purification MeSH
- Bacterial Typing Techniques MeSH
- Ubiquinone analogs & derivatives analysis MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- coenzyme Q10 MeSH Browser
- DNA, Bacterial MeSH
- Hexachlorocyclohexane * MeSH
- Soil Pollutants MeSH
- Fatty Acids MeSH
- RNA, Ribosomal, 16S MeSH
- Spermidine MeSH
- Ubiquinone MeSH
A yellow-pigmented bacterial strain, designated LL02(T), was isolated from hexachlorocyclohexane-contaminated soil from Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL02(T) occupied a distinct phylogenetic position in the genus Novosphingobium and showed the highest sequence similarity with Novosphingobium resinovorum NCIMB 8767(T) (98.59 %). DNA-DNA relatedness between strain LL02(T) and its closest phylogenetic neighbours was <70 %, which indicated that strain LL02(T) represented a novel species of the genus Novosphingobium. The DNA G+C content of strain LL02(T) was 67.72±0 mol%. The major respiratory quinone was ubiquinone Q-10. The polar lipid profile of the isolate corresponded to those reported for other members of the genus Novosphingobium (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and sphingoglycolipids), thus supporting its classification in the genus. Spermidine was the major polyamine. The major fatty acids were summed feature 3 (consisting of C(16 : 1)ω7c and/or C(16 : 1)ω6c; 40.13 %), summed feature 8 (consisting of C(18 : 1)ω7c and/or C(18 : 1)ω6c; 31.09 %) and C(14 : 0) 2-OH (23.16 %). The results obtained from DNA-DNA hybridization and biochemical and physiological tests clearly distinguished the isolate from its closest phylogenetic neighbours. Thus, strain LL02(T) represents a novel species of the genus Novosphingobium, for which the name Novosphingobium barchaimii sp. nov. is proposed. The type strain is LL02(T) ( = CCM 7980(T) = DSM 25411(T)).
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GENBANK
JN695619
