Imaging of protein synthesis: in vitro and in vivo evaluation of (44)Sc-DOTA-puromycin
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
- MeSH
- heterocyklické sloučeniny monocyklické chemie farmakokinetika farmakologie MeSH
- kinetika MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- molekulární zobrazování metody MeSH
- nádorové buněčné linie MeSH
- pozitronová emisní tomografie MeSH
- proteiny analýza chemie metabolismus MeSH
- proteosyntéza MeSH
- puromycin analogy a deriváty farmakokinetika farmakologie MeSH
- skandium chemie farmakokinetika MeSH
- tkáňová distribuce MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid MeSH Prohlížeč
- heterocyklické sloučeniny monocyklické MeSH
- proteiny MeSH
- puromycin MeSH
- skandium MeSH
PURPOSE: The purpose of this study was to investigate whether (44)Sc-labeled puromycin can be utilized for imaging of protein synthesis in vivo. METHODS: For micro-positron emission tomographic (μPET) studies, 20-25 MBq of [(44)Sc]-DOTA-puromycin was administered to tumor-bearing rats, and animals were scanned for 1 h dynamically. Results were further validated by dissecting organs and tissues of the animals after the measurement and in vitro blocking experiments using puromycin or cycloheximide to block protein synthesis. RESULTS: μPET images of tumor-bearing rats showed significant tumor uptake of [(44)Sc]-DOTA-puromycin and a clear-cut tumor visualization. In both blocking experiments, cellular uptake of [(44)Sc]-DOTA-puromycin ([(44)Sc]-DOTA-Pur) could be suppressed by blocking protein synthesis. CONCLUSIONS: We report for the first time successful μPET imaging with (44)Sc obtained from a (44)Ti/(44)Sc generator, as well as noninvasive μPET imaging of ribosomal activity, respectively protein synthesis, with a puromycin-based radiopharmaceutical and the direct correlation between cellular uptake of [(44)Sc]-DOTA-Pur and protein synthesis.
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