Four simple sets for digital microphotography are described that have been tested with the Carl Zeiss Jena, Meopta Prague, Lambda Prague, and LOMO Sankt Petersburg microscopes and with DSLR Nikon D 70 and Nikon D 300 cameras. They permit precise image focusing in the camera using a prism Zeiss. The sets make use of commonly available extensions Zeiss, Praktica and reductions Nikon-Praktica manufactured by ROWI (without a lens) or HAMA (with a lens). An extension has further been designed and manufactured for connecting the DSLR Nikon D 300 camera fitted with the HAMA reduction (only with a lens) and a focusing extensible prism with Zeiss Jena light measurement. It permits a precise image focusing of low light intensity objects (autofluorescence or low-contrast or moving objects when using positive or negative phase contrast). The sets are applicable to all microscopes constructed according to German DIN industrial standards.

Laboratory of Molecular Structure Characterization Institute of Microbiology Academy of Sciences of the Czech Republic Prague Czech Republic

Zobrazit více v PubMed

Folia Microbiol (Praha). 2006;51(2):109-13 PubMed

Folia Microbiol (Praha). 2010 Nov;55(6):625-8 PubMed

Folia Microbiol (Praha). 2001;46(6):495-503 PubMed

Proc Biol Sci. 2004 Jun 22;271(1545):1251-62 PubMed

Folia Microbiol (Praha). 2009 Sep;54(5):463-7 PubMed

Concomitant use of polarization and negative phase contrast microscopy for the study of microorganisms

Effect of metal ions on autofluorescence of the dry rot fungus Serpula lacrymans grown on spruce wood

Concomitant use of polarization and positive phase contrast microscopy for the study of microbial cells

Anisotropic structures of some microorganisms studied by polarization microscopy