Improvement in β-cell function after diet-induced weight loss is associated with decrease in pancreatic polypeptide in subjects with type 2 diabetes
Language English Country United States Media print-electronic
Document type Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't
PubMed
22673566
DOI
10.1016/j.jdiacomp.2012.05.003
PII: S1056-8727(12)00119-5
Knihovny.cz E-resources
- MeSH
- Insulin-Secreting Cells metabolism MeSH
- Models, Biological MeSH
- C-Peptide blood MeSH
- Exercise MeSH
- Diabetes Mellitus, Type 2 blood complications physiopathology MeSH
- Down-Regulation * MeSH
- Weight Loss MeSH
- Body Mass Index MeSH
- Combined Modality Therapy MeSH
- Middle Aged MeSH
- Humans MeSH
- Overweight complications diet therapy therapy MeSH
- Obesity complications diet therapy therapy MeSH
- Pancreatic Polypeptide blood metabolism MeSH
- Protein Precursors blood metabolism MeSH
- Diet, Reducing * MeSH
- Oxygen Consumption MeSH
- Life Style MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Clinical Trial MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- C-Peptide MeSH
- Pancreatic Polypeptide MeSH
- PPY protein, human MeSH Browser
- Protein Precursors MeSH
OBJECTIVE: The aim of our study was to evaluate the effect of a lifestyle intervention program on β-cell function and to explore the role of gastrointestinal peptides in subjects with T2D. METHODS: Subjects with T2D (n=74) received 24 weeks of intervention: 12 weeks of slimming diet (-500 kcal/day) and the subsequent 12 weeks of diet were combined with aerobic exercise. All subjects were examined at weeks 0, 12 and 24. β-cell function was assessed during standard meal tests. Insulin secretory rate (ISR) was calculated by C-peptide deconvolution, and β-cell function was quantified with a mathematical model. Plasma concentrations of gastrointestinal peptides were measured in a fasting state and during hyperinsulinemia induced by hyperinsulinemic isoglycemic clamp. RESULTS: Mean weight loss was 5.03±4.38 kg (p<0.001) in weeks 0-12. Weight did not change significantly in weeks 12-24. Both insulin secretion at the reference level and glucose sensitivity increased in weeks 0-12 (by 33%±54% and by 26%±53%, respectively, p<0.001) and remained unchanged in weeks 12-24. Both fasting and hyperinsulinemic plasma concentrations of pancreatic polypeptide (PP) decreased in weeks 0-12 (p<0.05 for both) and did not change significantly in weeks 12-24. Changes in insulin secretion at the reference level correlated negatively with plasma concentrations of PP during hyperinsulinemia (r=-0.36; p<0.001). Changes in glucose sensitivity correlated negatively with changes in plasma concentrations of PP, both in fasting and during hyperinsulinemia (r=-0.2; p=0.01 for both). The correlations remained significant after adjustment for changes in body-mass-index. CONCLUSIONS: After diet-induced weight loss, β-cell function improved in T2D subjects and remained unchanged after the addition of exercise. We demonstrate for the first time that these changes are associated with a decrease in PP secretion.
References provided by Crossref.org
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