Phylogenetic classification of Pleurothecium and Pleurotheciella gen. nov. and its dactylaria-like anamorph (Sordariomycetes) based on nuclear ribosomal and protein-coding genes
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22684295
DOI
10.3852/12-035
PII: 12-035
Knihovny.cz E-resources
- MeSH
- Ascomycota classification cytology genetics isolation & purification MeSH
- DNA, Fungal chemistry genetics MeSH
- Wood microbiology MeSH
- Fungal Proteins genetics MeSH
- Phylogeny * MeSH
- DNA, Ribosomal Spacer chemistry genetics MeSH
- Molecular Sequence Data MeSH
- Multilocus Sequence Typing MeSH
- Mycological Typing Techniques MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA Polymerase II genetics MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Fresh Water MeSH
- Spores, Fungal classification cytology genetics isolation & purification MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Fungal MeSH
- Fungal Proteins MeSH
- DNA, Ribosomal Spacer MeSH
- DNA, Ribosomal MeSH
- RNA Polymerase II MeSH
Two strains of an unidentified perithecial ascomycete with a dactylaria-like anamorph and another morphologically similar strain of a dactylaria-like fungus were collected on decaying wood submerged in freshwater. To study their phylogenetic relationships we (i) combined sequence data from the nuclear small and large subunits ribosomal DNA (nc18S and nc28S) and the second largest subunit of RNA polymerase II (RPB2) for a multigene phylogenetic analysis and (ii) used sequences of the internal transcribed spacer region (ITS) of the rRNA operon for a species-level analysis. The new genus Pleurotheciella is described for two new species, Pla. rivularia and Pla. centenaria, with nonstromatic perithecia, unitunicate asci, persistent paraphyses and hyaline, septate ascospores and dactylaria-like anamorphs characterized by holoblastic, denticulate conidiogenesis, subhyaline conidiophores and hyaline, septate conidia. Based on morphological and molecular data, Pleurotheciella is closely related to the genera Pleurothecium and Sterigmatobotrys. A key to the three genera and the known species is provided. In the three-gene inferred phylogeny, these genera grouped as a sister clade to the Savoryellales within a robust clade of uncertain higher rank affiliation. Phylogenetic study of the 12 strains that represent Pleurothecium recurvatum revealed four that grouped apart from the core of the species. Two of these strains, which form a monophyletic well supported clade in both phylogenies and share similar morphological characteristics, are described as a new species, Pleurothecium semifecundum.
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GENBANK
JQ429145, JQ429146, JQ429147, JQ429148, JQ429149, JQ429150, JQ429151, JQ429152, JQ429153, JQ429154, JQ429155, JQ429156, JQ429157, JQ429158, JQ429159, JQ429160, JQ429161, JQ429230, JQ429231, JQ429232, JQ429233, JQ429234, JQ429235, JQ429236, JQ429237, JQ429238, JQ429239, JQ429240, JQ429241, JQ429242, JQ429243, JQ429244, JQ429245, JQ429246, JQ429247, JQ429248, JQ429249, JQ429250, JQ429251, JQ429252, JQ429253, JQ429254, JQ429255, JQ429256, JQ429257, JQ429258, JQ429259, JQ429260, JQ429261, JQ429262, JQ429263, JQ429264, JQ429265, JQ429266, JQ429267, JQ429268, JQ429269, JQ429270, JQ429271, JQ429272, JQ437438, JQ437439
