Effect of tick saliva on signalling pathways activated by TLR-2 ligand and Borrelia afzelii in dendritic cells
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Phosphatidylinositol 3-Kinase metabolism MeSH
- Borrelia burgdorferi Group immunology MeSH
- Dendritic Cells immunology MeSH
- Immunologic Factors metabolism MeSH
- Interleukin-10 metabolism MeSH
- Ixodes pathogenicity MeSH
- Mitogen-Activated Protein Kinases metabolism MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Proto-Oncogene Proteins c-akt metabolism MeSH
- Signal Transduction drug effects MeSH
- Saliva metabolism MeSH
- Tumor Necrosis Factor-alpha metabolism MeSH
- Toll-Like Receptor 2 immunology MeSH
- Transcription Factor RelA metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phosphatidylinositol 3-Kinase MeSH
- Immunologic Factors MeSH
- Interleukin-10 MeSH
- Mitogen-Activated Protein Kinases MeSH
- Proto-Oncogene Proteins c-akt MeSH
- Tumor Necrosis Factor-alpha MeSH
- Toll-Like Receptor 2 MeSH
- Transcription Factor RelA MeSH
Dendritic cells are a sentinel in defending against pathogens and tick saliva facilitates transmission of tick-borne pathogens by modulating the host immune response. The maturation of dendritic cells is inhibited by tick saliva. To elucidate the mechanism of this inhibition, we tested the impact of Ixodes ricinus tick saliva on signalling pathways activated by Toll-like receptor (TLR-2) ligand and Borrelia afzelii in spleen dendritic cells. The activation of nuclear factor-κB (NF-κB) p65 and phosphatidylinositol-3 kinase (PI3K)/Akt pathways was decreased by tick saliva upon both TLR-2 and Borrelia stimulation. Among the mitogen-activated protein kinases (MAPK), the activation of extracellular matrix-regulated kinase (Erk1/2) was suppressed by tick saliva, but not p38. In response to spirochaetes, the amount of TNF-α decreased in the presence of tick saliva which was mediated by selective suppression of Erk1/2, NF-κB and Akt as tick saliva mimicked the effect of their specific inhibitors, UO126, IKK-IV and LY294002, respectively. Saliva-induced enhancement of IL-10 was not observed in the presence of specific inhibitor of Protein Kinase A (PKA), H-89, suggesting the involvement of PKA pathway in IL-10 production. Our cumulative data show that tick saliva interferes with several signalling pathways, thus modulating the immune functions of dendritic cells.
References provided by Crossref.org
For Whom the Bell Tolls (and Nods): Spit-acular Saliva
Modulation of host immunity by tick saliva
Tick sialostatins L and L2 differentially influence dendritic cell responses to Borrelia spirochetes
