"Click & seed" approach to the biomimetic modification of material surfaces
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biomimetics * MeSH
- Cyclization MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Surface Properties MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A simple, versatile, protein-repulsive, substrate-independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow-up modifications with various ligands using a copper-catalyzed alkyne-azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of (125)I-radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line.
References provided by Crossref.org
Fluorescent nanodiamonds embedded in biocompatible translucent shells
Nonfouling poly(ethylene oxide) layers end-tethered to polydopamine
