Vhc1, a novel transporter belonging to the family of electroneutral cation-Cl(-) cotransporters, participates in the regulation of cation content and morphology of Saccharomyces cerevisiae vacuoles
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23022132
DOI
10.1016/j.bbamem.2012.09.019
PII: S0005-2736(12)00340-9
Knihovny.cz E-resources
- MeSH
- Chlorides chemistry MeSH
- DNA chemistry MeSH
- Electrochemistry methods MeSH
- Microscopy, Fluorescence methods MeSH
- Phylogeny MeSH
- Genotype MeSH
- Ion Transport MeSH
- Cations chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Microscopy, Confocal methods MeSH
- Membrane Potentials MeSH
- Nigericin pharmacology MeSH
- Oligonucleotides chemistry MeSH
- Osmotic Pressure MeSH
- Open Reading Frames MeSH
- Recombination, Genetic MeSH
- Saccharomyces cerevisiae Proteins chemistry physiology MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Sorbitol chemistry MeSH
- Symporters chemistry physiology MeSH
- Vacuoles chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chlorides MeSH
- DNA MeSH
- Cations MeSH
- Nigericin MeSH
- Oligonucleotides MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Sorbitol MeSH
- Symporters MeSH
- VHC1 protein, S cerevisiae MeSH Browser
Cation-Cl(-) cotransporters (CCCs) are integral membrane proteins which catalyze the coordinated symport of Cl(-) with Na(+) and/or K(+) ions in plant and mammalian cells. Here we describe the first Saccharomyces cerevisiae CCC protein, encoded by the YBR235w open reading frame. Subcellular localization studies showed that this yeast CCC is targeted to the vacuolar membrane. Deletion of the YBR235w gene in a salt-sensitive strain (lacking the plasma-membrane cation exporters) resulted in an increased sensitivity to high KCl, altered vacuolar morphology control and decreased survival upon hyperosmotic shock. In addition, deletion of the YBR235w gene in a mutant strain deficient in K(+) uptake produced a significant growth advantage over the parental strain under K(+)-limiting conditions, and a hypersensitivity to the exogenous K(+)/H(+) exchanger nigericin. These results strongly suggest that we have identified a novel yeast vacuolar ion transporter mediating a K(+)-Cl(-) cotransport and playing a role in vacuolar osmoregulation. Considering its identified function, we propose to refer to the yeast YBR235w gene as VHC1 (vacuolar protein homologous to CCC family 1).
References provided by Crossref.org