The aim of the study was to further elucidate the immunohistochemical and genetic characteristics of cribriform adenocarcinoma of minor salivary glands (CAMSG). The study comprised five CAMSG from two males and three females, aged 21-72 years. Four tumors were localized at the base of tongue and one in the floor of mouth. At the time of diagnosis, four tumors had metastasised to regional lymph nodes. After tumor resection, two patients were treated by radiotherapy and one by chemoradiotherapy. During the follow-up (median 14 months), two patients developed lymph node metastasis. Microscopically, all tumors showed cribriform, papillary, follicular, and microcystic growth patterns. The tumor cells displayed vesicular nuclei with intranuclear grooves. Immunohistochemically, all tumors showed expression of cytokeratin (CK) 7, CK8, CK18, vimentin, smooth muscle actin, calponin, S-100 protein, and p16 protein. In addition, we observed expression of galectin-3, CK19, and HBME-1, but not of thyroglobulin and TTF-1. No mutations of RET, BRAF, K-RAS, H-RAS, and N-RAS proto-oncogenes were detected. However, in RET proto-oncogene, we found polymorphisms Gly691Ser (exon 11) and Ser904Ser (exon 15) in one case, p.Leu769Leu (exon 13) in one case, and variant p.IVS14-24 G/A of intron 14 in two cases, and in H-RAS proto-oncogene we found polymorphism 81 T-C (exon 1) in three cases. Thyroglobulin and TTF-1 are the only useful markers in the differential diagnosis between CAMSG and papillary thyroid carcinoma as both tumors may express galectin-3, CK19, and HBME-1. The RET, H-RAS, and N-RAS proto-oncoogenes are not mutated in CAMSG.

The Fingerland Department of Pathology Charles University Faculty of Medicine and Faculty Hospital Sokolska 581 500 05 Hradec Králové Czech Republic

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Cesk Patol. 2008 Oct;44(4):103-7 PubMed

Head Neck Pathol. 2011 Mar;5(1):51-6 PubMed

Endocr Pathol. 2010 Sep;21(3):178-85 PubMed

Am J Surg Pathol. 2011 Aug;35(8):1168-76 PubMed

Histopathology. 1998 Jun;32(6):521-9 PubMed

Expert Rev Mol Diagn. 2008 Jan;8(1):83-95 PubMed

Oncogene. 2011 May 5;30(18):2087-97 PubMed

Ear Nose Throat J. 2004 Jan;83(1):62-4 PubMed

J Endocrinol. 2004 Nov;183(2):257-65 PubMed

J Clin Endocrinol Metab. 2003 Jun;88(6):2745-52 PubMed

Int J Gynecol Pathol. 2007 Oct;26(4):475-80 PubMed

Histopathology. 1999 Dec;35(6):495-501 PubMed

J Oral Maxillofac Surg. 1989 Jan;47(1):86-8 PubMed

Arch Otolaryngol. 1983 Dec;109(12):827-31 PubMed

Oral Oncol. 1998 Sep;34(5):391-5 PubMed

Cancer Genet Cytogenet. 1991 Apr;52(2):187-91 PubMed

Mod Pathol. 2012 Jan;25(1):26-35 PubMed

Leukemia. 2003 Dec;17(12):2257-317 PubMed

Am J Surg Pathol. 2005 Nov;29(11):1530-6 PubMed

Oncol Lett. 2011 Jan;2(1):135-138 PubMed

Med Oncol Tumor Pharmacother. 1992;9(3):113-7 PubMed

Int J Biol Markers. 2007 Oct-Dec;22(4):239-44 PubMed

Int J Biochem Cell Biol. 2005 May;37(5):961-76 PubMed

Oncogene. 2006 Aug 3;25(33):4620-7 PubMed

Thyroid. 2008 Dec;18(12):1269-76 PubMed

Clin Endocrinol (Oxf). 2006 Jan;64(1):105-9 PubMed

Cancer. 2005 Jun 1;103(11):2261-8 PubMed

Pol J Pathol. 2011 Sep;62(3):168-71 PubMed

Int J Mol Sci. 2012;13(1):221-39 PubMed

J Endocrinol Invest. 2010 May;33(5):318-24 PubMed

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