SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23401421
DOI
10.1002/term.1694
Knihovny.cz E-zdroje
- Klíčová slova
- 2-hydroxyethyl methacrylate, IKVAV (Ile-Lys-Val-Ala-Val) peptide, hydrogel, oriented pores, scaffold, spinal cord repair,
- MeSH
- axony patologie MeSH
- biokompatibilní materiály chemie MeSH
- hojení ran MeSH
- hydrogely chemie MeSH
- krysa rodu Rattus MeSH
- mechanický stres MeSH
- mezenchymální kmenové buňky cytologie MeSH
- mikroskopie elektronová rastrovací MeSH
- oligopeptidy chemie MeSH
- polyhydroxyethylmethakrylát chemie MeSH
- poranění míchy rehabilitace terapie MeSH
- poréznost MeSH
- potkani Wistar MeSH
- pružnost MeSH
- regenerace nervu MeSH
- tkáňové inženýrství metody MeSH
- tkáňové podpůrné struktury chemie MeSH
- tlak MeSH
- zelené fluorescenční proteiny chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biokompatibilní materiály MeSH
- hydrogely MeSH
- oligopeptidy MeSH
- polyhydroxyethylmethakrylát MeSH
- seryl-isoleucyl-lysyl-valyl-alanyl-valinamide MeSH Prohlížeč
- zelené fluorescenční proteiny MeSH
The architecture and mechanical properties of a scaffold for spinal cord injury treatment must provide tissue integration as well as effective axonal regeneration. Previous work has demonstrated the cell-adhesive and growth-promoting properties of the SIKVAV (Ser-Ile-Lys-Val-Ala-Val)-modified highly superporous poly(2-hydroxethyl methacrylate) (PHEMA) hydrogels. The aim of the current study was to optimize the porosity and mechanical properties of this type of hydrogel in order to develop a suitable scaffold for the repair of spinal cord tissue. Three types of highly superporous PHEMA hydrogels with oriented pores of ~60 µm diameter, porosities of 57-68% and equivalent stiffness characterized by elasticity moduli in the range 3-45 kPa were implanted into a spinal cord hemisection, and their integration into the host tissue, as well as the extent of axonal ingrowth into the scaffold pores, were histologically evaluated. The best tissue response was found with a SIKVAV-modified PHEMA hydrogel with 68% porosity and a moderate modulus of elasticity (27 kPa in the direction along the pores and 3.6 kPa in the perpendicular direction). When implanted into a spinal cord transection, the hydrogel promoted tissue bridging as well as aligned axonal ingrowth. In conclusion, a prospective oriented scaffold architecture of SIKVAV-modified PHEMA hydrogels has been developed for spinal cord injury repair; however, to develop an effective treatment for spinal cord injury, multiple therapeutic approaches are needed.
Department of Neuroscience 2nd Medical Faculty Charles University Prague Czech Republic
Department of Neurosurgery Masaryk Hospital Ústí nad Labem Czech Republic
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
Citace poskytuje Crossref.org
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