Focal cerebral ischemia induces the neurogenic potential of mouse Dach1-expressing cells in the dorsal part of the lateral ventricles
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23458709
DOI
10.1016/j.neuroscience.2013.02.048
PII: S0306-4522(13)00189-9
Knihovny.cz E-resources
- MeSH
- 4-Aminopyridine pharmacology MeSH
- Sodium Channel Blockers pharmacology MeSH
- Cell Differentiation physiology MeSH
- Nerve Degeneration etiology pathology MeSH
- Adult Stem Cells physiology MeSH
- Infarction, Middle Cerebral Artery complications MeSH
- Membrane Potentials drug effects MeSH
- Patch-Clamp Techniques MeSH
- Disease Models, Animal MeSH
- Mice, Inbred C57BL MeSH
- Mice, Transgenic MeSH
- Mice MeSH
- Neurogenesis physiology MeSH
- Neurons metabolism MeSH
- Eye Proteins metabolism MeSH
- Cell Count MeSH
- Nerve Tissue Proteins metabolism MeSH
- In Vitro Techniques MeSH
- Tetraethylammonium pharmacology MeSH
- Tetrodotoxin pharmacology MeSH
- Lateral Ventricles pathology MeSH
- Green Fluorescent Proteins genetics MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 4-Aminopyridine MeSH
- Sodium Channel Blockers MeSH
- Dach1 protein, mouse MeSH Browser
- Eye Proteins MeSH
- Nerve Tissue Proteins MeSH
- Tetraethylammonium MeSH
- Tetrodotoxin MeSH
- Green Fluorescent Proteins MeSH
The mouse Dach1 gene, involved in the development of the neocortex and the hippocampus, is expressed by neural stem cells (NSCs) during early neurogenesis, and its expression also continues in a subpopulation of cells in the dorsal part of the lateral ventricles (LV) of the adult mouse brain. In this study we aimed to elucidate the role of Dach1-expressing cells in adult neurogenesis/gliogenesis under physiological as well as post-ischemic conditions, employing transgenic mice in which the expression of green fluorescent protein (GFP) is controlled by the D6 promotor of the mouse Dach1 gene. A neurosphere-forming assay of GFP⁺ cells isolated from the dorsal part of the LV was carried out with subsequent differentiation in vitro. To elucidate the neurogenic/gliogenic potential of GFP⁺ cells in the dorsal part of the LV, in situ immunohistochemical/electrophysiological analyses of GFP⁺ cells in adult sham-operated brains (controls) and those after middle cerebral artery occlusion (MCAo) were performed. The GFP⁺ cells isolated from the dorsal part of the LV of controls formed neurospheres and differentiated solely into a glial phenotype, while those isolated after MCAo also gave rise to cells with the properties of neuronal precursors. In situ analyses revealed that GFP⁺ cells express the phenotype of adult NSCs or neuroblasts in controls as well as following ischemia. Following MCAo we found a significantly increased number of GFP⁺ cells expressing doublecortin as well as a number of GFP⁺ cells migrating through the rostral migratory stream into the olfactory bulb, where they probably differentiated into calretinin⁺ interneurons. Collectively, our results suggest the involvement of the mouse Dach1 gene in adult neurogenesis; cells expressing this gene exhibit the properties of adult NSCs or neuroblasts and respond to MCAo by enhanced neurogenesis.
References provided by Crossref.org
Heterogeneity of astrocytes: from development to injury - single cell gene expression
