Focal cerebral ischemia induces the neurogenic potential of mouse Dach1-expressing cells in the dorsal part of the lateral ventricles
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23458709
DOI
10.1016/j.neuroscience.2013.02.048
PII: S0306-4522(13)00189-9
Knihovny.cz E-zdroje
- MeSH
- 4-aminopyridin farmakologie MeSH
- blokátory sodíkových kanálů farmakologie MeSH
- buněčná diferenciace fyziologie MeSH
- degenerace nervu etiologie patologie MeSH
- dospělé kmenové buňky fyziologie MeSH
- infarkt arteria cerebri media komplikace MeSH
- membránové potenciály účinky léků MeSH
- metoda terčíkového zámku MeSH
- modely nemocí na zvířatech MeSH
- myši inbrední C57BL MeSH
- myši transgenní MeSH
- myši MeSH
- neurogeneze fyziologie MeSH
- neurony metabolismus MeSH
- oční proteiny metabolismus MeSH
- počet buněk MeSH
- proteiny nervové tkáně metabolismus MeSH
- techniky in vitro MeSH
- tetraethylamonium farmakologie MeSH
- tetrodotoxin farmakologie MeSH
- ventriculi laterales patologie MeSH
- zelené fluorescenční proteiny genetika MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 4-aminopyridin MeSH
- blokátory sodíkových kanálů MeSH
- Dach1 protein, mouse MeSH Prohlížeč
- oční proteiny MeSH
- proteiny nervové tkáně MeSH
- tetraethylamonium MeSH
- tetrodotoxin MeSH
- zelené fluorescenční proteiny MeSH
The mouse Dach1 gene, involved in the development of the neocortex and the hippocampus, is expressed by neural stem cells (NSCs) during early neurogenesis, and its expression also continues in a subpopulation of cells in the dorsal part of the lateral ventricles (LV) of the adult mouse brain. In this study we aimed to elucidate the role of Dach1-expressing cells in adult neurogenesis/gliogenesis under physiological as well as post-ischemic conditions, employing transgenic mice in which the expression of green fluorescent protein (GFP) is controlled by the D6 promotor of the mouse Dach1 gene. A neurosphere-forming assay of GFP⁺ cells isolated from the dorsal part of the LV was carried out with subsequent differentiation in vitro. To elucidate the neurogenic/gliogenic potential of GFP⁺ cells in the dorsal part of the LV, in situ immunohistochemical/electrophysiological analyses of GFP⁺ cells in adult sham-operated brains (controls) and those after middle cerebral artery occlusion (MCAo) were performed. The GFP⁺ cells isolated from the dorsal part of the LV of controls formed neurospheres and differentiated solely into a glial phenotype, while those isolated after MCAo also gave rise to cells with the properties of neuronal precursors. In situ analyses revealed that GFP⁺ cells express the phenotype of adult NSCs or neuroblasts in controls as well as following ischemia. Following MCAo we found a significantly increased number of GFP⁺ cells expressing doublecortin as well as a number of GFP⁺ cells migrating through the rostral migratory stream into the olfactory bulb, where they probably differentiated into calretinin⁺ interneurons. Collectively, our results suggest the involvement of the mouse Dach1 gene in adult neurogenesis; cells expressing this gene exhibit the properties of adult NSCs or neuroblasts and respond to MCAo by enhanced neurogenesis.
Citace poskytuje Crossref.org
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