Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23579777
DOI
10.1039/c3cc41438h
Knihovny.cz E-resources
- MeSH
- DNA Primers metabolism MeSH
- DNA-Directed DNA Polymerase metabolism MeSH
- Guanosine Triphosphate chemistry metabolism MeSH
- Metallocenes MeSH
- Nitrobenzenes chemistry MeSH
- Nucleotides chemistry MeSH
- Oligonucleotides biosynthesis chemistry MeSH
- Oxidation-Reduction MeSH
- Ferrous Compounds chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Primers MeSH
- DNA-Directed DNA Polymerase MeSH
- ferrocene MeSH Browser
- Guanosine Triphosphate MeSH
- Metallocenes MeSH
- nitrobenzene MeSH Browser
- Nitrobenzenes MeSH
- Nucleotides MeSH
- Oligonucleotides MeSH
- Ferrous Compounds MeSH
Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.
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