A comparative study of meiotic recombination in cattle (Bos taurus) and three wildebeest species (Connochaetes gnou, C. taurinus taurinus and C. t. albojubatus)
Language English Country Switzerland Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
23594414
DOI
10.1159/000350444
PII: 000350444
Knihovny.cz E-resources
- MeSH
- Centromere genetics MeSH
- In Situ Hybridization, Fluorescence MeSH
- Meiosis * MeSH
- Pachytene Stage MeSH
- Sex Chromosomes genetics MeSH
- Ruminants genetics MeSH
- Cell Cycle Proteins genetics MeSH
- Recombination, Genetic * MeSH
- Chromosomes, Mammalian genetics MeSH
- Cattle genetics MeSH
- Spermatocytes cytology MeSH
- Synaptonemal Complex genetics MeSH
- Testis cytology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Cattle genetics MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Cell Cycle Proteins MeSH
The karyotypic evolution in the family Bovidae is based on centric fusions of ancestral acrocentric chromosomes. Here, the frequency and distribution of meiotic recombination was analyzed in pachytene spermatocytes from Bos taurus (2n = 60) and 3 wildebeest species (Connochaetes gnou, C. taurinus taurinus and C. t. albojubatus) (2n = 58) using immunofluorescence and fluorescence in situ hybridization. Significant differences in mean numbers of recombination events per cell were observed between B. taurus and members of the genus Connochaetes (47.2 vs. 43.7, p < 0.001). The number of MLH1 foci was significantly correlated with the length of the autosomal synaptonemal complexes. The average interfocus distance was influenced by interference. The male recombination maps of bovine chromosomes 2 and 25 and of their fused homologues in wildebeests were constructed. A significant reduction of recombination in the fused chromosome BTA25 was observed in wildebeests (p = 0.005). This was probably caused by interference acting across the centromere, which was significantly stronger than the intra-arm interference. This comparative meiotic study showed significant differences among the species from the family Bovidae with the same fundamental number of autosomal arms (FNa = 29) which differ by a single centric fusion.
References provided by Crossref.org
Meiotic behaviour of evolutionary sex-autosome translocations in Bovidae