A new cultivation-independent tool for fast and reliable detection of Mycobacterium marinum
Language English Country Great Britain, England Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
23634824
DOI
10.1111/jfd.12113
Knihovny.cz E-resources
- Keywords
- bacterial typing, cutaneous infection, fish mycobacteriosis, molecular microbiology, real-time PCR, zoonosis,
- MeSH
- Mycobacterium Infections, Nontuberculous diagnosis microbiology MeSH
- Bacterial Proteins genetics MeSH
- Bacteriological Techniques methods standards MeSH
- Molecular Diagnostic Techniques methods standards MeSH
- Species Specificity MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Humans MeSH
- Multiplex Polymerase Chain Reaction MeSH
- Mycobacterium marinum genetics isolation & purification MeSH
- Fish Diseases diagnosis microbiology MeSH
- Nontuberculous Mycobacteria genetics isolation & purification MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
The Mycobacterium marinum group (MMG) is a class of mycobacteria that includes M. marinum, the cause of chronic systemic infections in fish. This species occasionally causes granulomatous skin lesions in humans. Other members of MMG are mycolactone-producing mycobacteria (MPM; M. ulcerans, M. shottsii and M. pseudoshottsii). The cultivation-independent approach presented in this study brings a fast and reliable alternative to classically used cultivation methods. The developed triplex erp/IS2404 qPCR assay is based on a primary species-specific erp detection, which allows enumeration of MMG in analysed samples, and secondary IS2404 detection is suitable for the differentiation of M. marinum from MPM. The detection of M. marinum in clinical specimens and in artificially contaminated tissue samples has proven its applicability for diagnostic purposes.
References provided by Crossref.org
Fish tank granuloma caused by Mycobacterium marinum in two aquarists: two case reports
