Identification of bifidobacteria isolated from Asian elephant (Elephas maximus)
Jazyk angličtina Země Indie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- bakteriální RNA genetika MeSH
- Bifidobacterium genetika růst a vývoj izolace a purifikace MeSH
- DNA fingerprinting MeSH
- feces mikrobiologie MeSH
- fenotyp MeSH
- fermentace MeSH
- molekulární typizace MeSH
- polymerázová řetězová reakce MeSH
- RNA ribozomální 16S genetika MeSH
- sloni mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální RNA MeSH
- RNA ribozomální 16S MeSH
Bifidobacteria are considered as one of the key genera in intestinal tracts of animals, and their species composition vary depending on the host. The aim of this study was to identify faecal bifidobacteria from Asian elephants (Elephas maximus), housed in Zoological gardens (Ostrava, Czech Republic). Using culturing, bifidobacteria were found in counts 7.60+/-0.56 log CFU/g. Twenty-six pure strains were isolated from faeces of Asian elephant. The isolates were clustered into two groups according to fingerprinting profiles and fermentation characteristic. Bacteria were identified by a combination of MALDI-TOF MS, PCR methods and sequencing as B. boum (12 isolates) and B. adolescentis (14 isolates). Elephant strains showed different fingerprinting profiles than type and collection strains. Since these two species are frequently isolated from gastrointestinal tract of herbivores, they seem to be typical of animals fed plant diets.
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