Usefulness of PCR-HRMA in identification of non-fermentative Gram-negative rods recovered from patients suffering from cystic fibrosis or chronic obstructive pulmonary disease
Language English Country United States Media print-electronic
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacteriological Techniques methods MeSH
- Pulmonary Disease, Chronic Obstructive complications MeSH
- Cystic Fibrosis complications MeSH
- Molecular Diagnostic Techniques methods MeSH
- DNA, Bacterial chemistry genetics MeSH
- Gram-Negative Aerobic Rods and Cocci genetics isolation & purification MeSH
- Gram-Negative Bacterial Infections diagnosis microbiology MeSH
- Respiratory Tract Infections microbiology MeSH
- Humans MeSH
- Polymerase Chain Reaction methods MeSH
- Prospective Studies MeSH
- Transition Temperature MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
Adequate treatment of microbial infections requires rapid and accurate identification of the etiological agent. In routine diagnostics, identification of bacteria conventionally relies on phenotypic testing, which can be hindered by phenotypic variations. Therefore, genotyping techniques should perform faster and more accurately. Recently, the technique of high-resolution melting analysis (HRMA) of PCR amplicons promises to provide a convenient and economic tool of genotypic identification. In our study, we performed prospective routine testing of a PCR-HRMA system that was recently published in a proof-of-the-principle study. The system was evaluated by analysing 275 clinical isolates of bacteria acquired from 65 patients suffering from cystic fibrosis or chronic obstructive pulmonary disease. Our results show that its routine use may result in partial worsening of its discriminatory power; however, it still outmatched conventional phenotyping in the group of non-fermentative Gram-negative rods. Moreover, when supplemented by rapid, simple and economic oxidase test, it can be even simplified for more economic performance.
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