Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo
Language English Country Slovakia Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23817638
DOI
10.4149/gpb_2013031
Knihovny.cz E-resources
- MeSH
- Child MeSH
- Adult MeSH
- Down-Regulation drug effects radiation effects MeSH
- Epilobium chemistry MeSH
- Erythema drug therapy etiology MeSH
- Extracellular Matrix drug effects metabolism radiation effects MeSH
- Phenotype MeSH
- Fibroblasts cytology drug effects metabolism radiation effects MeSH
- Fibronectins genetics MeSH
- GPI-Linked Proteins genetics MeSH
- Hyaluronoglucosaminidase genetics MeSH
- Skin cytology drug effects radiation effects MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Cell Adhesion Molecules genetics MeSH
- Radiation-Protective Agents chemistry pharmacology therapeutic use MeSH
- Gene Expression Regulation drug effects radiation effects MeSH
- Plant Extracts chemistry pharmacology therapeutic use MeSH
- Connective Tissue Growth Factor genetics MeSH
- Sirtuin 1 genetics MeSH
- Cellular Senescence drug effects radiation effects MeSH
- Skin Aging drug effects radiation effects MeSH
- Ultraviolet Rays adverse effects MeSH
- Cell Survival drug effects radiation effects MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CCN2 protein, human MeSH Browser
- Fibronectins MeSH
- GPI-Linked Proteins MeSH
- Hyal2 protein, human MeSH Browser
- Hyaluronoglucosaminidase MeSH
- Cell Adhesion Molecules MeSH
- Radiation-Protective Agents MeSH
- Plant Extracts MeSH
- Connective Tissue Growth Factor MeSH
- SIRT1 protein, human MeSH Browser
- Sirtuin 1 MeSH
Stress-induced fibroblast senescence is thought to contribute to skin aging. Ultraviolet light (UV) radiation is the most potent environmental risk factor in these processes. An Epilobium angustifolium (EA) extract was evaluated for its capacity to reverse the senescent response of normal human dermal fibroblasts (NHDF) in vitro and to exhibit skin photo-protection in vivo. The HPLC-UV-MS analysis of the EA preparation identified three major polyphenol groups: tannins (oenothein B), phenolic acids (gallic and chlorogenic acids) and flavonoids. EA extract increased the cell viability of senescent NHDF induced by serum deprivation. It diminished connective tissue growth factor and fibronectin gene expressions in senescent NHDF. Down-regulation of the UV-induced release of both matrix metalloproteinase-1 and -3 and the tissue inhibitor of matrix metalloproteinases-1 and -2, and also down-regulation of the gene expression of hyaluronidase 2 were observed in repeatedly UV-irradiated NHDF after EA extract treatment. Interestingly, EA extract diminished the down-regulation of sirtuin 1 dampened by UV-irradiation. The application of EA extract using a sub-irritating dose protected skin against UV-induced erythema formation in vivo. In summary, EA extract diminished stress-induced effects on NHDF, particularly on connective tissue growth factor, fibronectin and matrix metalloproteinases. These results collectively suggest that EA extract may possess anti-aging properties and that the EA polyphenols might account for these benefits.
References provided by Crossref.org
Initial cell adhesion of three cell types in the presence and absence of serum proteins
