HI-6 modulates immunization efficacy in a BALB/c mouse model
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23958973
DOI
10.1016/j.etap.2013.07.012
PII: S1382-6689(13)00159-2
Knihovny.cz E-resources
- Keywords
- Acetylcholine receptor, Acetylcholinesterase, Antibody, Cholinergic anti-inflammatory pathway, Inflammation, Vaccine,
- MeSH
- Adjuvants, Immunologic pharmacology MeSH
- Antidotes pharmacology MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Freund's Adjuvant MeSH
- Hemocyanins immunology MeSH
- Immunity drug effects MeSH
- Immunization * MeSH
- Interleukin-6 analysis biosynthesis MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Oximes pharmacology MeSH
- Antibodies analysis MeSH
- Pyridinium Compounds pharmacology MeSH
- Th1-Th2 Balance drug effects MeSH
- Antibody Formation drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Antidotes MeSH
- asoxime chloride MeSH Browser
- Freund's Adjuvant MeSH
- Hemocyanins MeSH
- Interleukin-6 MeSH
- keyhole-limpet hemocyanin MeSH Browser
- Oximes MeSH
- Antibodies MeSH
- Pyridinium Compounds MeSH
HI-6 is used as an antidote to nerve agents. It can also act as an antagonist to acetylcholine receptors (AChRs) including the nicotinic receptor, α 7 nAChR which is involved in regulating the immune response through macrophages. This experiment investigated the efficacy of HI-6 to regulate the immune response. Laboratory BALB/c mice received HI-6 and/or keyhole limpet hemocyanin (KLH) as an antigen. Antibody production was investigated after either 21 or 65 days when either single or repeated dose of antigen was applied. We confirmed that HI-6 significantly improved vaccination efficacy when KLH was given in a dose of 1mg/kg. The effect was dose dependent. A combination of HI-6 and KLH produced a vaccination of almost the same efficacy as that for Freund's complete adjuvant. The findings point at the suitability of HI-6 for improving vaccination efficacy at the level of immunity regulation by the nervous system.
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