Diversity of chlorobiphenyl-metabolizing bacteria and their biphenyl dioxygenases in contaminated sediment
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24007621
DOI
10.1016/j.chemosphere.2013.07.073
PII: S0045-6535(13)01057-6
Knihovny.cz E-zdroje
- Klíčová slova
- 16S rRNA genes analysis, Bacteria metabolizing PCBs, Biphenyl dioxygenase, Stable isotope probing,
- MeSH
- Bacteria klasifikace genetika metabolismus MeSH
- biodegradace MeSH
- dioxygenasy genetika metabolismus MeSH
- fylogeneze MeSH
- genetická variace MeSH
- geologické sedimenty chemie mikrobiologie MeSH
- látky znečišťující půdu analýza metabolismus MeSH
- molekulární sekvence - údaje MeSH
- polychlorované bifenyly analýza metabolismus MeSH
- půdní mikrobiologie MeSH
- sekvence nukleotidů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dioxygenasy MeSH
- látky znečišťující půdu MeSH
- polychlorované bifenyly MeSH
Bacteria and bacterial communities in sites contaminated with polychlorinated biphenyls have been extensively studied in the past decades. However, there are still major gaps in the knowledge of environmental processes, especially in the behavior of previously described bacteria in vitro, their real degradation abilities and the enzymes that are involved in the degradation processes. In this work we analyzed actively degrading bacterial populations by stable isotope probing with (13)C biphenyl and (13)C-4-chlorobiphenyl as labeled substrates in the environment of sediment contaminated with polychlorinated biphenyls. We performed analysis of populations which degrade biphenyl and 4-chlorobiphenyl at concentrations similar to those of the original site. Several bacterial genera were revealed to actively participate in biphenyl and 4-chlorobiphenyl removal, some of which had not previously been described to take part in this process. We also found there are few differences in the communities metabolizing biphenyl and 4-chlorobiphenyl. Analysis of the genes responsible for substrate removal proved most of the genes to be closely related to Pseudomonas pseudoalcaligenes KF707 genes giving bacteria the ability of transforming di-para-chlorinated biphenyls.
Citace poskytuje Crossref.org
Pseudomonads Rule Degradation of Polyaromatic Hydrocarbons in Aerated Sediment
GENBANK
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