Diversity of chlorobiphenyl-metabolizing bacteria and their biphenyl dioxygenases in contaminated sediment
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24007621
DOI
10.1016/j.chemosphere.2013.07.073
PII: S0045-6535(13)01057-6
Knihovny.cz E-resources
- Keywords
- 16S rRNA genes analysis, Bacteria metabolizing PCBs, Biphenyl dioxygenase, Stable isotope probing,
- MeSH
- Bacteria classification genetics metabolism MeSH
- Biodegradation, Environmental MeSH
- Dioxygenases genetics metabolism MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Geologic Sediments chemistry microbiology MeSH
- Soil Pollutants analysis metabolism MeSH
- Molecular Sequence Data MeSH
- Polychlorinated Biphenyls analysis metabolism MeSH
- Soil Microbiology MeSH
- Base Sequence MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Dioxygenases MeSH
- Soil Pollutants MeSH
- Polychlorinated Biphenyls MeSH
Bacteria and bacterial communities in sites contaminated with polychlorinated biphenyls have been extensively studied in the past decades. However, there are still major gaps in the knowledge of environmental processes, especially in the behavior of previously described bacteria in vitro, their real degradation abilities and the enzymes that are involved in the degradation processes. In this work we analyzed actively degrading bacterial populations by stable isotope probing with (13)C biphenyl and (13)C-4-chlorobiphenyl as labeled substrates in the environment of sediment contaminated with polychlorinated biphenyls. We performed analysis of populations which degrade biphenyl and 4-chlorobiphenyl at concentrations similar to those of the original site. Several bacterial genera were revealed to actively participate in biphenyl and 4-chlorobiphenyl removal, some of which had not previously been described to take part in this process. We also found there are few differences in the communities metabolizing biphenyl and 4-chlorobiphenyl. Analysis of the genes responsible for substrate removal proved most of the genes to be closely related to Pseudomonas pseudoalcaligenes KF707 genes giving bacteria the ability of transforming di-para-chlorinated biphenyls.
References provided by Crossref.org
Pseudomonads Rule Degradation of Polyaromatic Hydrocarbons in Aerated Sediment
GENBANK
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