Plasma membrane density of GABA(B)-R1a, GABA(B)-R1b, GABA-R2 and trimeric G-proteins in the course of postnatal development of rat brain cortex
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24020808
DOI
10.33549/physiolres.932552
PII: 932552
Knihovny.cz E-resources
- MeSH
- Cell Membrane metabolism MeSH
- Heterotrimeric GTP-Binding Proteins metabolism MeSH
- Rats MeSH
- Cerebral Cortex growth & development metabolism MeSH
- GTP-Binding Protein alpha Subunit, Gi2 metabolism MeSH
- GTP-Binding Protein alpha Subunits, Gi-Go metabolism MeSH
- GTP-Binding Protein alpha Subunits metabolism MeSH
- GTP-Binding Protein beta Subunits metabolism MeSH
- Receptors, GABA-B metabolism MeSH
- Sodium-Potassium-Exchanging ATPase metabolism MeSH
- Age Factors MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Gabbr2 protein, rat MeSH Browser
- Gnai1 protein, rat MeSH Browser
- Gnai2 protein, rat MeSH Browser
- Gnai3 protein, rat MeSH Browser
- Gnaz protein, rat MeSH Browser
- Heterotrimeric GTP-Binding Proteins MeSH
- GTP-Binding Protein alpha Subunit, Gi2 MeSH
- GTP-Binding Protein alpha Subunits, Gi-Go MeSH
- GTP-Binding Protein alpha Subunits MeSH
- GTP-Binding Protein beta Subunits MeSH
- Receptors, GABA-B MeSH
- Sodium-Potassium-Exchanging ATPase MeSH
With the aim to understand the onset of expression and developmental profile of plasma membrane (PM) content /density of crucial components of GABA(B)-R signaling cascade, GABA(B)-R1a, GABA(B)-R1b, GABA(B)-R2, G(i)1/G(i)2alpha, G(i)3alpha, G(o)alpha, G(z)alpha and Gbeta subunit proteins were determined by quantitative immunoblotting and compared in PM isolated from brain cortex of rats of different ages: between postnatal-day-1 (PD1) and 90 (PD90). PM density of GABA(B)-R1a, GABA(B)-R2, G(i)1/G(i)2alpha, G(i)3alpha, G(o)alpha, G(z)alpha and Gbeta was high already at birth and further development was reflected in parallel decrease of both GABA(B)-R1a and GABA(B)-R2 subunits. The major decrease of GABA(B)-R1a and GABA(B)-R2 occurred between the birth and PD15: to 55 % (R1a, **) and 51 % (R2, **), respectively. Contrarily, PM level of the cognate G-proteins G(i)1/G(i)2alpha, G(i)3alpha, G(o)alpha, G(z)alpha and Gbeta was unchanged in the course of the whole postnatal period of brain cortex development. Maturation of GABA(B)-R cascade was substantially different from ontogenetic profile of prototypical plasma membrane marker, Na, K-ATPase, which was low at birth and further development was reflected in continuous increase of PM density of this enzyme. Major change occurred between the birth and PD25. In adult rats, membrane content of Na, K-ATPase was 3-times higher than around the birth.
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