This work is concerned with the surface modification of fluorescent silica nanoparticles by a monoclonal antibody (M75) and the specific bioadhesion of such particles to surfaces containing the PG domain of carbonic anhydrase IX (CA IX), which is a trans-membrane protein specifically expressed on the surfaces of several tumor cell lines. The adhesion strength of antibody-bearing silica nanoparticles to antigen-bearing surfaces was investigated under laminar flow conditions in a microfluidic cell and compared to the adhesion of unmodified silica nanoparticles and nanoparticles coupled with an unspecific antibody. Adhesion to cancer cells using flow cytometry was also investigated and in all cases the adhesion strength of M75-modified nanoparticles was significantly stronger than for the unmodified or unspecific nanoparticles, up to several orders of magnitude in some cases. The specific modification of nano- and microparticles by an antibody-like protein therefore appears to be a feasible approach for the targeting of tumor cells.

Institute of Chemical Technology Prague Department of Chemical Engineering Technická 3 Prague 6 166 28 Czech Republic

Zobrazit více v PubMed

Čejková J., Stěpánek F. Curr. Pharm. Des. 2013;19:6298–6314. PubMed

Zadrazil A., Tokarova V., Stepanek F. Soft Matter. 2012;8:1811–1816.

Tokarova V., Pittermannova A., Cech J., Ulbrich P., Stepanek F. Soft Matter. 2012;8:1087–1095.

Tokárová V., Kašpar O., Knejzlík Z., Ulbrich P., Štěpánek F. Powder Technol. 2013;235:797–805.

Haufova P., Dohnal J., Hanus J., Stepanek F. Colloids Surf., A. 2012;410:52–58.

Vasir J. K., Tambwekar K., Garg S. Int. J. Pharm. 2003;255:13–32. PubMed

Guillemot G., Lorthois S., Schmitz P., Mercier-Bonin M. Chem. Eng. Res. Des. 2007;85:800–807.

Prabhakarpandian B., Shen M. C., Pant K., Kiani M. F. Microvasc. Res. 2011;82:210–220. PubMed PMC

Doshi N., Prabhakarpandian B., Rea-Ramsey A., Pant K., Sundaram S., Mitragotri S. J. Controlled Release. 2010;146:196–200. PubMed PMC

Jain R. K., Stylianopoulos T. Nat. Rev. Clin. Oncol. 2010;7:653–664. PubMed PMC

Maeda H. Adv. Enzyme Regul. 2001;41:189–207. PubMed

Funakoshi N., Onizuka M., Yanagi K., Ohshima N., Tomoyasu M., Sato Y., Yamamoto T., Ishikawa S., Mitsui T. Microvasc. Res. 2000;59:361–367. PubMed

Hansen-Algenstaedt N., Joscheck C., Schaefer C., Lamszus K., Wolfram L., Biermann T., Algenstaedt P., Brockmann M. A., Heintz C., Fiedler W., Ruther W. Eur. J. Cancer. 2005;41:1073–1085. PubMed

Yuan F., Dellian M., Fukumura D., Leunig M., Berk D. A., Torchilin V. P., Jain R. K. Cancer Res. 1995;55:3752–3756. PubMed

Tanaka M., Komagata M., Tsukada M., Kamiya H. Powder Technol. 2008;183:273–281.

Ott M. L., Mizes H. A. Colloids Surf., A. 1994;87:245–256.

Zhang H. A., Ding W. Q., Law K. Y., Cetinkaya C. Powder Technol. 2011;208:582–589.

Blackwell J. E., Dagia N. M., Dickerson J. B., Berg E. L., Goetz D. J. Ann. Biomed. Eng. 2001;29:523–533. PubMed

Abbassi O., Kishimoto T. K., Mcintire L. V., Anderson D. C., Smith C. W. J. Clin. Invest. 1993;92:2719–2730. PubMed PMC

Kral V., Mader P., Collard R., Fabry M., Horejsi M., Rezacova P., Kozisek M., Zavada J., Sedlacek J., Rulisek L., Brynda J. Proteins. 2008;71:1275–1287. PubMed

Gieling R. G., Williams K. J. Bioorg. Med. Chem. 2013;21:1470–1476. PubMed

Kivela A. J., Knuuttila A., Rasanen J., Sihvo E., Salmenkivi K., Saarnio J., Pastorekova S., Pastorek J., Waheed A., Sly W. S., Salo J. A., Parkkila S. Bioorg. Med. Chem. 2013;21:1483–1488. PubMed

Zavada J., Zavadova Z., Machon O., Kutinova L., Opavsky R., Pastorek J. Int. J. Oncol. 1997;10:857–863. PubMed

Zavada J., Zavadova Z., Pastorek J., Biesova Z., Jezek J., Velek J. Br. J. Cancer. 2000;82:1808–1813. PubMed PMC

Stöber W., Fink A., Bohn E. J. Colloid Interface Sci. 1968;26:62–69.

Cejkova J., Hanus J., Stepanek F. J. Colloid Interface Sci. 2010;346:352–360. PubMed

Karg M., Pastoriza-Santos I., Liz-Marzan L. M., Hellweg T. ChemPhysChem. 2006;7:2298–2301. PubMed

Vasir J. K., Labhasetwar V. Biomaterials. 2008;29:4244–4252. PubMed PMC

Pyo N., Tanaka S., McNamee C. E., Kanda Y., Fukumori Y., Ichikawa H., Higashitani K. Colloids Surf., B. 2006;53:278–287. PubMed

Shinto H., Aso Y., Fukasawa T., Higashitani K. Colloids Surf., B. 2012;91:114–121. PubMed

Jain R. K. Cancer Res. 1988;48:2641–2658. PubMed

Kang S. T., Subramani A., Hoek E. M. V., Deshusses M. A., Matsumoto M. R. J. Membr. Sci. 2004;244:151–165.

Kiani M. F., Yuan H., Chen X., Smith L., Gaber M. W., Goetz D. J. Pharm. Res. 2002;19:1317–1322. PubMed

Liang S., Slattery M. J., Dong C. Exp. Cell Res. 2005;310:282–292. PubMed PMC

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Antibody-pHPMA functionalised fluorescent silica nanoparticles for colorectal carcinoma targeting