Use of fluorescent staining and flow cytometry for monitoring physiological changes in solventogenic clostridia
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24211310
DOI
10.1016/j.anaerobe.2013.10.006
PII: S1075-9964(13)00173-X
Knihovny.cz E-zdroje
- Klíčová slova
- Clostridium beijerinckii, Clostridium tetanomorphum, Flow cytometry, Fluorescence staining,
- MeSH
- barbituráty MeSH
- barvení a značení metody MeSH
- bioreaktory MeSH
- Clostridium fyziologie ultrastruktura MeSH
- fermentace * MeSH
- fluoresceiny MeSH
- fluorescenční barviva MeSH
- isoxazoly MeSH
- propidium MeSH
- průtoková cytometrie MeSH
- techniky vsádkové kultivace MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- barbituráty MeSH
- bis(1,3-dibutylbarbiturate)trimethine oxonol MeSH Prohlížeč
- carboxyfluoresceindiacetate MeSH Prohlížeč
- fluoresceiny MeSH
- fluorescenční barviva MeSH
- isoxazoly MeSH
- propidium MeSH
Physiological changes in populations of Clostridium beijerinckii and Clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. To estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to be a good choice for both species. During stationary phase, these stains did not reflect physiological changes sufficiently and therefore additional labeling with bis-(1,3-dibutylbarbituric acid) trimethineoxonol was applied. Results of fluorescence staining in solventogenic batch fermentations were compared with substrate-use data, the concentration of key metabolites and growth curves. We demonstrate that measurements by all methods were mutually compatible.
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