Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24333335
DOI
10.1016/j.febslet.2013.11.038
PII: S0014-5793(13)00885-5
Knihovny.cz E-resources
- Keywords
- 1,6-diphenyl-1,3,5-hexatriene, DHS, DL-dihydrosphingosine, DPH, Fluidity, Fluorescence anisotropy, Lipid order, Membrane microdomain, Time-resolved fluorescence, all-trans-9,11,13,15-octadecatetraenoic acid, t-PnA,
- MeSH
- Cell Membrane chemistry metabolism MeSH
- Diphenylhexatriene chemistry MeSH
- Fluorescence Polarization MeSH
- Spectrometry, Fluorescence MeSH
- Membrane Microdomains chemistry metabolism MeSH
- Mutation MeSH
- Fatty Acids, Unsaturated chemistry MeSH
- Saccharomyces cerevisiae MeSH
- Sphingolipids biosynthesis chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Diphenylhexatriene MeSH
- Fatty Acids, Unsaturated MeSH
- parinaric acid MeSH Browser
- Sphingolipids MeSH
We report sphingolipid-related reorganization of gel-like microdomains in the plasma membrane of living Saccharomyces cerevisiae using trans-Parinaric acid (t-PnA) and 1,6-diphenyl-1,3,5-hexatriene (DPH). Compared to control, the gel-like domains were significantly reduced in the membrane of a sphingolipid-deficient lcb1-100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin-induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less-ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids.
References provided by Crossref.org
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